By means of synthetic biology techniques, the chassis cells of Escherichia coli are engineered to construct engineered bacteria with the functions of "pathogen detection, directional synthesis, and ecological adaptation", so as to achieve the efficient synthesis of erucamide.
In our project, to prevent and control tomato bacterial wilt caused by Ralstonia solanacearum, we have developed two sets of system components, namely the fatty acid induction system and the erucamide production sensing system. The fatty acid induction system responds to the glycolytic system, initiates fatty acid synthesis, and activates the downstream erucamide production sensing system.

In the fatty acid induction system: Since the production of fatty acids requires the PtsG gene (BBa_25SJHD2N) to rapidly accumulate acetyl-CoA through glycolysis, and to induce the expression of FabH (BBa_25ROJHES) (thereby initiating fatty acid synthesis), we have conducted the expression (with BBa_25HKBP2Y and BBa_25YH73O6) and protein purification of these two genes, thus activating our fatty acid induction system.
In the erucamide production sensing system: The synthesis of erucamide depends on an amidation reaction, with the reaction formula as follows: Erucic acid + Glutamine → Erucamide + Glutamic acid. GlnA (BBa_25GZZ1FM) provides the amide group donor (glutamine) required for the reaction during erucamide production, acting as the direct nitrogen source for erucamide synthesis. Meanwhile, it maintains the intracellular glutamine pool and supports the activity of fatty acid amide hydrolases FAA1/FAA2. Therefore, we have performed the expression (with BBa_25X7OHDK) and protein purification of this gene, thereby activating our erucamide production sensing system.