General Tasks:

Fundraising, Safety, Project Administration

Specific tasks:

(1) Safety: went over experimental protocols with iGEM team and ensured they complied with the safety guidelines or requirements of iGEM. (2) Fundraising: facilitated conversation between iGEM and the BioE financial department to secure access to team funding sources. (3) Project Administration: Facilitated the recruitment of the 2025 Stanford iGEM Team

General Tasks:

Entrepreneurship, Public Engagement, Fundraising, Project Administration

Specific tasks:

(1) Entrepreneurship: utilized her unique background as an entrepreneur to aid in development of entrepreneurial efforts (2) Project Administration: introduced project management systems such as the "Kanban board" and "Scrum" to help the project efficiently identify, manage, and execute competition requirements. Facilitated the recruitment of the 2025 Stanford iGEM Team (3) Public Engagement: gave advice on how to structure and present writing material as well as how to reach out and seek the wider scientific community, science communication in general. (4) Fundraising: helped us identify and facilitate contact between our team and funding sources; developed initial team budget.

General Tasks:

Safety, Investigation, Writing, Public Engagement, Visualization, Project Administration

Specific tasks:

(1) Investigation: acted as the de-facto consultant for any science-related questions that iGEM had. Attended mentor meetings and provided advice on the science aspect of our project (e.g. identifying how an experiment went wrong, sharing protocols and lab tips/tricks), helped the team troubleshoot and analyze data. (2) Safety: taught iGEM members how to perform certain experiments (e.g. electroporation, sterile technique) safely. (3) Public Engagement: gave advice on how to structure and present writing material as well as how to reach out and seek the wider scientific community, science communication in general. (4) Writing: assisted in reviewing and providing feedback for Wiki writing. (5) Visualization: provided feedback on data visualization figures. (6) Project Administration: Facilitated the recruitment of the 2025 Stanford iGEM Team

General Tasks:

Project Administration, Investigation, Safety, Public Engagement, Visualization

Specific tasks:

(1) Project Administration: Facilitated the recruitment of the 2025 Stanford iGEM Team. (2) Investigation: acted as the de-facto consultant for any science-related questions that iGEM had. Attended mentor meetings and provided advice on the science aspect of our project (e.g. identifying how an experiment went wrong, sharing protocols and lab tips/tricks), helped the team troubleshoot and analyze data. (3) Safety: taught iGEM members how to perform certain experiments safely and navigate the UTL laboratory spaces (4) Public Engagement: gave advice on how to structure and present writing material as well as how to reach out and seek the wider scientific community, science communication in general. (5) Writing: assisted in reviewing and providing feedback for Wiki writing. (6) Visualization: provided feedback on data visualization figures.

General Tasks:

Analysis, Conceptualization, Background Research, Project Administration, Public Engagement, Software, Wiki Coding, Visualization, Writing

Specific tasks:

(1) Liz developed Python pipelines for thermodynamic and kinetic modeling of RADAR sensors, integrating ΔG-based scoring with RNAcofold, batch sequence processing, and Michaelis–Menten/Lineweaver–Burk fitting to rank triggers and visualize editing efficiencies. (2) She co-led early design with Melwin, comparing CRISPR and paper-based diagnostics before defining RADAR as the core system for HBV-induced hepatocellular carcinoma. (3) Liz reviewed the 2023 Nature Biotechnology RADAR paper, Gao Lab updates, and transcriptomic data from GEO and GTEx. (4) She managed the team budget, coordinated Paris Jamboree logistics, and maintained Notion tracking. (5) For Human Practices and Education, Liz organized TurBiohacks, a 36-hour hackathon with NTHU, NUS, and IIT Madras, and led collaboration with THICA through bilingual interviews with founder Jacki Chen. (6) She built the wiki's navigation in HTML/CSS/JavaScript, wrote content, and created illustrations and animations explaining RADAR logic.

General Tasks:

Analysis, Conceptualization, Background Research, Investigation, Project Administration, Public Engagement, Entrepreneurship, FundraisingVisualization, Writing, Safety

Specific tasks:

(1) Melwin was the Outreach and Education Lead and contributed across wet lab, dry lab, entrepreneurship, and Human Practices. (2) He analyzed cancer databases such as TCGA to identify biomarkers, co-led early project ideation with Liz to define RADAR, and reviewed literature on liver cancer and therapeutic outputs, identifying candidate proteins for validation. (3) In the wet lab, Melwin designed and transfected plasmids to evaluate RADAR AND gate performance using split GFP and IL-2. (4) He coordinated team operations, managed supply orders, and secured a $2000 ZYMO Research grant. (5) Melwin led outreach with academic and industry mentors including Pheast Therapeutics, Genesis, and AbbVie, organizing a mentorship with Pheast and co-leading SIBRP 2025. (6) He also supported entrepreneurship meetings, created slides and posters, contributed to wiki and safety forms, and coordinated lab cleanup with Uytengsu Lab staff.

General Tasks:

Analysis, Conceptualization, Background Research, Investigation, Data Curation, Notebook/record keeping, Project Administration, Visualization, Writing, Public Engagement

Specific tasks:

(1) Analysis – Analyzed Hepatitis B viral sequences from NCBI to identify conserved HBx regions for RADAR sensor design, compared Hep3B cell line data with viral variants, and optimized sensor assemblies. (2) Conceptualization – Contributed to developing the RADAR biosensor concept by identifying conserved viral sequences and manually editing sensor sequences. (3) Investigation – Wrote and optimized all wet lab protocols, developed the summer experimental calendar, and led laboratory efforts. (4) Data Curation – Maintained detailed records of experiments, compiled results, and created figures comparing expected versus observed interactions. (5) Visualization – Designed visual materials illustrating background, results, and sequence interactions. (6) Writing – Authored all wet lab wiki sections and registered plasmids in the iGEM Parts Registry. (7) Public Engagement – Mentored students from Egypt and Ethiopia to establish a synthetic biology non-profit.

General Tasks:

Analysis, Conceptualization, Background Research, Investigation, Project Administration, Public Engagement, Visualization, Writing, Safety

Specific tasks:

Bernardo served as the team's Experiment Lead, designing the system and developing experimental workflows connecting Wet Lab and Dry Lab. He led therapeutic trigger identification while working closely with medical school faculty to guide project design. Bernardo personally carried out extensive wet lab work including cloning, cell culture, and plasmid design, implementing and troubleshooting experimental workflows. He coordinated the team's partnership with RADAR Therapeutics for future collaboration on scaling the technology. Beyond the lab, Bernardo ideated TurBioHaks, a 36-hour international virtual synthetic biology hackathon with NTHU, NUS, and IIT Madras, and contributed to team communications and outreach.

General Tasks:

Analysis, Software, Investigation, Background Research, Public Engagement, Writing, Other

Specific tasks:

Annabelle was the head of programming support for our team. (1) Programming – She wrote scripts to rank endogenous and viral triggers, as well as characterize viral RNA counts in cell lines. (2) Investigation – She sourced data on Hepatocellular Carcinoma and Hepatitis B from public databases like Gepia2, GenBank, and DepMap. (3) Background Research – She performed multiple literature reviews of RNA folding software, multiple sequence alignment (MSA) algorithms, Hepatitis B (HepB)'s mechanism of infection, the truncation of HepB's HBX gene, and the relationship between liver cirrhosis and Hepatocellular Carcinoma. (4) Hackathon Coordination – Organized the Hackathon and Cortical Labs collaboration, did lecture series outreach, moderated the Hackathon server, and judged submissions. (5) Public Engagement – Presented on resume curation during SIBRP and helped with SIBRP admissions. (6) Writing – Assisted in the writing and editing of wiki pages.

General Tasks:

Analysis, Conceptualization, Background Research, Investigation, Data Curation, Software, Public Engagement, Writing

Specific tasks:

(1) Analysis – Analyzed cancer databases including TCGA and GEPIA2 to identify hepatocellular carcinoma over-expressed genes and processed HBV bulk RNA sequencing datasets. (2) Software – Programmed a pipeline that performs multiple sequence alignment across eight HBV genotypes to identify conserved regions and scans for CCA, GCA, and UCA motifs as potential biomarkers for the RADAR system, and assisted Anna with dry lab projects. (3) Background Research – Performed literature reviews on RNA folding software, modeling algorithms, ADAR binding mechanics, and HepB mechanisms. (4) Investigation – Cultured mammalian cells, performed transfections, imaging, and flow cytometry to evaluate AND gate performance using split GFP and split IL-2 outputs. (5) Data Curation – Collected transcriptomic data supporting dry lab modeling. (6) Project Administration – Tracked team progress and troubleshooted problems. (7) Public Engagement – Engaged with academic stakeholders, assisted with outreach and coordination efforts for IHP interviews, analyzed survey data, and delivered a bioengineering lecture for SIBRP. (8) Writing – Contributed to team video script, media, and wiki content.

General Tasks:

Public Engagement, Entrepreneurship, Investigation, Data Curation, Analysis, Safety, Visualization, Project Administration, Conceptualization, Writing

Specific tasks:

(1) Sophia led the Integrated Human Practices component and contributed across entrepreneurship, wet lab, analysis, and outreach. (2) She coordinated interviewers, conducted and documented interviews, and designed an IHP survey to guide project direction. (3) For entrepreneurship, with Rhea, Fatima, and Melwin, she built pitch decks, performed market sizing, interviewed experts, and joined weekly Pheast meetings. (4) In the lab, she performed cell culture, transfections, mini-preps, microscopy, and flow cytometry. (5) Sophia analyzed FlowJo data, generated histograms and fold-change plots, and ran statistical tests in Colab. (6) She compiled the Safety Form, taught biomolecules and college applications during SIBRP, and assisted with admissions. (7) She also created the BioRender workflow diagram for presentations, (8) ordered all plasmids from Twist, (9) joined ideation meetings, and (10) edited the Human Practices and Wet Lab wiki pages.

General Tasks:

Background Research, Project Administration, Public Engagement, Entrepreneurship, Software, Visualization, Writing

Specific tasks:

(1) Background Research – Researched optimal GC content range, favorable RNA secondary structure, and unique sequence nature after genome mapping while configuring criteria to rank RADAR trigger candidates. (2) Project Administration – Coordinated educational program logistics including reaching out to guest speakers, creating/editing slides, and co-managing the teaching assistant team. (3) Public Engagement – Maintained communication channels by scaling and managing the Stanford iGEM Instagram account, responding to collaboration requests and student interest. (4) Entrepreneurship – With Rhea, Sophia, and Melwin, developed pitch decks, approximated market size, interviewed experts, and sustained weekly communication with Pheast biotech mentor. (5) Software – Replicated and refined a sensor sequence ranking system from the Gao Lab, created an algorithm to find 140 conserved base pair windows across HBV genomes. (6) Visualization – Created supplemental visualization for 140 base pair conservation sequence tracking. (7) Writing – Helped write and edit content for the wiki.

General Tasks:

Background Research, Conceptualization, Investigation, Data Curation, Analysis, Project Administration, Entrepreneurship, Visualization, Writing

Specific tasks:

(1) Rhea led the Entrepreneurship component and contributed across wet-lab, dry-lab, and outreach. (2) Background Research and Analysis – analyzed online databases to identify conserved viral biomarker sequences, reviewed literature on HCC and therapeutic outputs, and found HBV-HCC bulk RNA seq data from online databases. (3) Data Curation - performed cell culture, transfections, mini-preps, microscopy, and flow cytometry for sensor and AND-gate validation. (4) Entrepreneurship – coordinated expert interviews with biotech and business professionals, developed the business model and commercialization strategy, organized pitch development, and researched the regulatory pathway and IP strategy. (5) Public Engagement – led synthetic bio workshops and bioethics lectures at GetSET and SIBRP, collaborated with Khan Lab students, and gave lab tours to high schoolers. (6) Visualization – created figures to explain the project and business components. (7) Writing – contributed to wiki and safety forms.

General Tasks:

Project Administration, Fundraising, Public Engagement, Safety, Writing

Specific tasks:

(1) Project Administration: Facilitated the recruitment of the 2025 Stanford iGEM Team, application process, and onboarding efforts (2) Fundraising: Coordinated with administration to apply for grants and secure team funding for project needs (3) Public Engagement: Provided auxiliary support and guidance for students in SIBRP (4) Writing: Assisted in reviewing and providing feedback for Wiki writing. (5) Safety: Ensured Stanford iGEM abided by Stanford's, iGEM, and the UTL safety regulations, provided iGEM members wet lab training

General Tasks:

Project Administration, Fundraising, Public Engagement, Safety, Writing

Specific tasks:

(1) Project Administration: Facilitated the recruitment of the 2025 Stanford iGEM Team, application process, and onboarding efforts (2) Fundraising: Coordinated with administration to apply for grants and secure team funding for project needs (3) Public Engagement: Provided auxiliary support and guidance for students in SIBRP (4) Writing: Assisted in reviewing and providing feedback for Wiki writing. (5) Safety: Ensured Stanford iGEM abided by Stanford's, iGEM, and the UTL safety regulations, provided iGEM members wet lab training

General Tasks:

Project Administration, Fundraising, Public Engagement, Safety, Writing

Specific tasks:

(1) Project Administration: Facilitated the recruitment of the 2025 Stanford iGEM Team, application process, and onboarding efforts (2) Fundraising: Coordinated with administration to apply for grants and secure team funding for project needs (3) Public Engagement: Provided auxiliary support and guidance for students in SIBRP (4) Writing: Assisted in reviewing and providing feedback for Wiki writing. (5) Safety: Ensured Stanford iGEM abided by Stanford's, iGEM, and the UTL safety regulations, provided iGEM members wet lab training

General Tasks:

Project Administration, Fundraising, Public Engagement, Safety, Writing

Specific tasks:

(1) Project Administration: Facilitated the recruitment of the 2025 Stanford iGEM Team, application process, and onboarding efforts (2) Fundraising: Coordinated with administration to apply for grants and secure team funding for project needs (3) Public Engagement: Provided auxiliary support and guidance for students in SIBRP (4) Writing: Assisted in reviewing and providing feedback for Wiki writing. (5) Safety: Ensured Stanford iGEM abided by Stanford's, iGEM, and the UTL safety regulations, provided iGEM members wet lab training

General Tasks:

Software, Safety, Public Engagement, Writing, Visualization

Specific tasks:

(1) Software: aided in the coding and debugging of our dry lab efforts; helped the team troubleshoot and analyze data. (2) Safety: went over experimental protocols with iGEM team and ensured they complied with the safety guidelines or requirements of iGEM. (3) Public Engagement: gave advice on how to structure and present writing material as well as how to reach out and seek the wider scientific community, science communication in general. (4) Writing: assisted in reviewing and providing feedback for Wiki writing. (5) Visualization: provided feedback on Wiki and team logo designs.

General Tasks:

Project Administration, Investigation, Writing, Public Engagement, Visualization

Specific tasks:

(1) Project Administration: Facilitated the recruitment of the 2025 Stanford iGEM Team. (2) Investigation: Attended mentor meetings and provided advice on the science aspect of our project (e.g. identifying how an experiment went wrong, sharing protocols and lab tips/tricks), helped the team troubleshoot and analyze data. (3) Public Engagement: gave advice on how to structure and present writing material as well as how to reach out and seek the wider scientific community, science communication in general. (4) Writing: assisted in reviewing and providing feedback for Wiki writing. (5) Visualization: provided feedback on data visualization figures.

General Tasks:

Project Administration, Fundraising, Public Engagement, Safety, Writing

Specific tasks:

(1) Project Administration: Facilitated the recruitment of the 2025 Stanford iGEM Team, application process, and onboarding efforts (2) Fundraising: Coordinated with administration to apply for grants and secure team funding for project needs (3) Public Engagement: Provided auxiliary support and guidance for students in SIBRP (4) Writing: Assisted in reviewing and providing feedback for Wiki writing. (5) Safety: Ensured Stanford iGEM abided by Stanford's, iGEM, and the UTL safety regulations, provided iGEM members wet lab training

General Tasks:

Conceptualization, Background Research

Specific tasks:

(1) Shared clinical perspectives on hepatocellular carcinoma (HCC) and its biomarkers. (2) Guided the team on experimental design and relevance of liver cancer models. (3) Connected the team to resources and expertise for wet lab work related to HBV-induced HCC.

General Tasks:

Investigation, Software, Other

Specific tasks:

(1) Developed the RADAR system on which the team's project is based, providing the foundational framework for programmable RNA sensing using ADAR editing. (2) Shared conceptual and technical guidance on how RADAR sensors can be adapted for disease detection and therapeutic applications. (3) Provided input on both dry lab modeling approaches and wet lab experimental design to ensure that the project remained aligned with the latest advances in RNA synthetic biology.

General Tasks:

Software, Investigation

Specific tasks:

(1) Wet Lab: provided feedback on cloning strategies, plasmid design, and experimental execution. (2) Dry Lab: advised on computational modeling approaches and helped refine the integration of RADAR sensor design with experimental validation.

General Tasks:

Dry Lab

Specific tasks:

(1) Gave advice on RNA structure prediction and computational modeling relevant to RADAR trigger design. (2) Assisted the team in evaluating modeling strategies and interpreting results for dry lab validation.

General Tasks:

Dry Lab

Specific tasks:

(1) Provided detailed suggestions on thermodynamic and kinetic modeling of RNA sensors. (2) Helped troubleshoot computational workflows for evaluating RADAR complex formation and editing efficiency.

Specific tasks:

Provided CRISPR educational kits for outreach and teaching activities, offered guidance on incorporating CRISPR-based materials into team workshops, and supported wet lab efforts by sharing resources and protocols relevant to gene editing education.

Description:

Initial project planning and research phase

Description:

Team formation and member selection

Description:

Experimental work and laboratory research

Description:

Computational modeling and data analysis

Description:

Website development and content creation