Wet Lab Description
Our experimental process in developing LiRA
Wet Lab Mission
The Wet Lab Team focused on designing and testing sensor and trigger sequences for key transcripts prominent in HBV-induced HCC, including HBx, GPC3, and AKR1B10. After validating individual RADAR sensors, we used them to construct a programmable AND-gate: combining GPC3 and HBx sensors with a split GFP reporter. Following characterization of the AND gate with the split GFP, we used the best versions for the therapeutic split IL-2 protein AND-gate output.