SAFETY

First, before the formal experiment began, all the team members participated in the safety training course. The team conducted experimental safety education together and watched safety education videos. During the experiment, all the laboratory personnel were clearly informed of the laboratory rules and were supervised by the safety advisor. When encountering experimental safety issues that they do not understand, team members can also raise questions to the consultant at any time and receive guidance. As mentioned above, our experimental process emphasizes safety and standardization, thereby ensuring the safety of the experiment.

Our biosafety committee consists with one leading teacher and two research assistants. They are primarily responsible for ensuring the members' safety and hygiene conditions of the work area. They offered specialized safety training for all team members to ensure a safe and correct working environment. They ensure our personal safety and the proper usage of equipment during experiments.

1. Strictly adhere to laboratory safety regulations, operational manuals, and standard procedures to ensure orderly experimental work.
2. Acquire thorough knowledge of the locations of utility pipelines, valves, switches, firefighting apparatus, emergency showers, eyewash stations, first-aid kits, and emergency cabinets, along with proficiency in emergency response protocols.
3. Unauthorized electrical modifications are strictly prohibited; maintain reliable insulation of wire connections, prevent circuit overloads, and deactivate power sources for equipment in prolonged disuse.
4. Wear nationally compliant personal protective equipment, including laboratory coats during all operations. Specialized gloves are mandatory for chemical or biological experiments. Unauthorized entry into non-laboratory zones is forbidden.
5. Experiments involving hazardous chemicals or substances require a minimum of two personnel. Operators must remain at their stations throughout procedures and promptly sanitize work surfaces upon completion.
6. Bulk storage of hazardous chemicals and waste is prohibited. Segregate chemicals by compatibility for isolated storage. Collect and contain hazardous waste separately.
7. Refrain from non-experimental activities including eating, drinking, smoking, cosmetic application, or resting. Laboratory access is restricted to authorized personnel exclusively.
8. Maintain impeccable laboratory cleanliness. Storage of extraneous items, clutter, or waste is prohibited. Ensure unobstructed evacuation routes and safety exits.
9. Terminate all utility services and restore equipment upon concluding experiments.
10. The final departing personnel must verify deactivation of utilities and secure all access points to guarantee laboratory integrity.
This protocol ensures personnel safety, prevents accidents, and maintains operational integrity.

General microbiology laboratory

Escherichia coli DH5α, Escherichia coli BL21, Escherichia coli, Nissle 1917

During the laboratory experiments, all participants must clearly define the emergency contact number and the designated person in charge. And during laboratory activities, all laboratory personnel need to wear standard personal protective equipment, such as laboratory work clothes, gloves, goggles, etc. And when encountering unfamiliar links, one should communicate with professional counselor.

The safety of the experimental process is of great significance. During team experiments, all team members should prioritize safety issues. We are very pleased to announce that our laboratory procedures comply with the code and regulations, are orderly and have no safety issues. Looking to the future, we will continue to strive to ensure the safety of experiments.