Molecular Lab

Molecular Clone

PCR Protocol(Q5 High-Fidelity Multiplex) PCR Protocol(Phanta UniFi Master Mix) PCR Protocol(Taq Master Mix) Agarose DNA Analysis of Gel Electrophoresis Protocol Nucleic Acid Purification Protocol EndoFree Plasmid Extraction Protocol Plasmid Extraction Protocol (TIANprep Mini Plasmid Kit) Genomic DNA Extraction Protocol Overlap Extension PCR Protocol Seamless Cloning Assembly Protocol Heat Shock Transformation Protocol Colony PCR Protocol

Gene Knockout

Electroporation-mediated Transformation Protocol Lambda RED homologous recombination method for Escherichia coli gene knockout Protocol

Other Molecular Experiments

Basic Microbiological Techniques Protocol Assessment of Biofilm Formation Protocol Enzyme-Linked Immunosorbent Assay (ELISA) Protocol Bac-Lumi™ Bacterial Firefly Luciferase Reporter Assay Protocol

Cell Lab

Cell Culture & Assays

Basic Manipulation of RAW 264.7 Macrophages Protocl Flow-Cytometric Protocol Enzyme-Linked Immunosorbent Assay (ELISA) Protocol