We have developed a new type of cream. We inactivate Saccharomyces cerevisiae, which already expresses antifreeze proteins and trehalose, by disrupting its cell, yielding a crude enzyme solution. We then found a suitable formula, combining this crude enzyme solution containing antifreeze proteins and trehalose with skincare ingredients that also have moisturizing properties. This cream forms a protective layer on the skin, directly inhibiting ice crystal growth and reducing physical damage caused by low temperatures. It also provides moisturizing benefits, providing skin protection for those working in cold environments.
Figure 2. Cream sample display