Overview

We have engineered a strain of Saccharomyces cerevisiae that secretes trehalose and, after induction, expresses antifreeze proteins. Our research has revealed that antifreeze proteins and trehalose possess numerous beneficial properties. We hope to apply these proteins to a wider range of applications, such as creams, door handle antifreeze agents, and leather protectants. The following sections will focus on these three application scenarios.

Cream

We have developed a new type of cream. We inactivate Saccharomyces cerevisiae, which already expresses antifreeze proteins and trehalose, by disrupting its cell, yielding a crude enzyme solution. We then found a suitable formula, combining this crude enzyme solution containing antifreeze proteins and trehalose with skincare ingredients that also have moisturizing properties. This cream forms a protective layer on the skin, directly inhibiting ice crystal growth and reducing physical damage caused by low temperatures. It also provides moisturizing benefits, providing skin protection for those working in cold environments.

Tianjin 2025 Model Framework Diagram
Figure 1. Schematic diagram of cream (front and back)
Tianjin 2025 Model Framework Diagram

Figure 2. Cream sample display
Tianjin 2025 Model Framework Diagram
Figure 3. Business Plan

Bio-antifreeze for concealed door handles

Concealed door handles are a modern automotive choice that better aligns with aerodynamic principles and aesthetic trends. However, in harsh winters, such handles can freeze and cause inconvenience during travel. To address this, we plan to apply antifreeze proteins to the anti-icing coating of concealed door handles. These antifreeze materials operate on an adsorption-inhibition mechanism, making it difficult for ice layers to form on the surface of the handles. This ensures the smooth operation of concealed door handles during winter, allowing people to open car doors more conveniently.

From the perspective of the "eco-friendly, efficient, and reliable" requirements of smart mobility, this new anti-icing coating solution addresses the pain points of traditional methods, such as "pollution, high energy consumption, and frequent maintenance." It aligns with the current trend in new energy vehicles that emphasizes "low cost and high environmental sustainability." In the future, we also aim to optimize the stability of antifreeze proteins in the door handle environment and enhance the adhesion of the antifreeze materials. We hope that this novel biomaterial will provide greater convenience for the public.

Tianjin 2025 Model Framework Diagram

Figure 4. Conceptual diagram of hidden door handle antifreeze coating

High-end leather product protective agent

We've noticed that high-end leather can become hard and crack in winter, so we're interested in applying our antifreeze proteins and trehalose to protect high-end leather from the cold, preserving its beauty and style. Our antifreeze proteins prevent ice crystals from forming, preventing the water in the leather from freezing and degrading its strength and appearance. Trehalose maintains the structural integrity of collagen, a core component of leather, and enhances its flexibility. Applying this protective agent to leather products like handbags and shoes would be crucial for maintaining their style and durability.

Tianjin 2025 Model Framework Diagram
Figure 5. Conceptual diagram of leather protectant