Overview
The catalog of TJUSLS_China in 2025 includes 40 new fundamental components and X improved parts. All components comply with the RFC[10] or RFC[1000] standard, have been functionally tested and recorded in the registry for community use.
New basic parts
We conducted enzyme activity tests using an orthogonal experimental design on the 40 protein sequences mined from an ocean database. Among them, we found that four proteins exhibit PET-degrading enzyme activity under certain conditions.
New Basic PET-Degrading Enzyme Parts Catalog
| Part Name | Name/Short Description | Part Type | ID Number in GOMC | Description | ||
|---|---|---|---|---|---|---|
| Optimum pH | Optimal NaCl Concentration/M | Optimum Temperature/℃ | ||||
| BBa_25ERRSY9 | B6 | Coding | >SRR8062318_GL0674422 | 8 | 4 | 50 |
| BBa_25NLL6X5 | B7 | Coding | >SRR7479623_GL0026367 | 8 | 4 | 50 |
| BBa_25A3MGA6 | B8 | Coding | >SRR100298_GL0002528 | 3 | ||
| BBa_25P0HHP3 | B14 | Coding | >SRR6207735_GL6219842 | 8 | 3 | 50 |
* Due to its relatively low melting temperature (Tm), the enzymatic activity of B8 decreases sharply with temperature changes. Consequently, its optimal NaCl concentration and optimum temperature were not determined in the experiment.
The remaining 36 protein sequences (mined from marine metagenomic data) show potential PET-degrading activity based on sequence homology and structural prediction. These sequences have been registered in the iGEM registry and are pending further functional verification.
Potential PET-Degrading Enzyme Sequences Catalog
| Part Name | Name/Short Description | Part Type | ID Number in GOMC |
|---|---|---|---|
| BBa_25GMA72J | A1 | Coding | >SRR100298_GL0001396 |
| BBa_25PCKO1E | A2 | Coding | >SRR5579847_GL1354857 |
| BBa_25NFYZDP | A3 | Coding | >SRR5248173_GL0931407 |
| BBa_25FYDH59 | A4 | Coding | >SRR8554435_GL1722417 |
| BBa_25UC942C | A5 | Coding | >SRR2657575_GL2081488 |
| BBa_25C9UP1W | A6 | Coding | >SRR8639428_GL0523750 |
| BBa_254ZAXAC | A7 | Coding | >SAMN03002195_gene_id_755445 |
| BBa_25152RFH | A8 | Coding | >SRR11563580_GL1648569 |
| BBa_25MKD4B7 | A9 | Coding | >SRR2657219_GL1767401 |
| BBa_25TOA4VR | A10 | Coding | >SRR7130820_GL2339226 |
| BBa_25TH86TK | A11 | Coding | >SRR11241203_GL2346779 |
| BBa_25F41NKP | A12 | Coding | >SRR8554434_GL1030282 |
| BBa_25ARWS05 | A13 | Coding | >SRR12358723_GL2364183 |
| BBa_25lL14V7 | A14 | Coding | >SRR7216773_GL17812797 |
| BBa_25KNDJO2 | A15 | Coding | >SRR8707046_GL0516124 |
| BBa_25EGBU2A | A16 | Coding | >SRR3933381.1.bin.8_02554 |
| BBa_250049NG | A17 | Coding | >SRR3933290.1.bin.10_00567 |
| BBa_254HSMXE | A18 | Coding | >SRR10276843_GL0046810 |
| BBa_25BKBBHC | A19 | Coding | >SAMN05422148_gene_id_18112 |
| BBa_25JATLMD | A20 | Coding | >SRR4458014_GL0003935 |
| BBa_25Z0GJX4 | B1 | Coding | >SRR7216773_GL0572691 |
| BBa_255N1N1X | B2 | Coding | >SRR11241201_GL5087216 |
| BBa_253XWMW | B3 | Coding | >SRR11434628_GL0055563 |
| BBa_25T8WGM | B4 | Coding | >ERR1679395_gene_id_255363 |
| BBa_257LMIS7 | B5 | Coding | >SRR5820075_GL4123555 |
| BBa_2590ICOE | B9 | Coding | >SRR10072860_GL8542186 |
| BBa_25OPF45U | B10 | Coding | >SRR12660002_GL0195430 |
| BBa_252YO2P4 | B11 | Coding | >SRR11922359_GL1654165 |
| BBa_25K906D4 | B12 | Coding | >SRR2657566_GL1786273 |
| BBa_25U7RPPE | B13 | Coding | >SRR6207735_GL1448155 |
| BBa_25SG9MB3 | B15 | Coding | >SRR7614707_GL0421034 |
| BBa_25CYXDDV | B16 | Coding | >SAMN14511311_gene_id_77418 |
| BBa_25S68L24 | B17 | Coding | >ERR598942_GL1124304 |
| BBa_25GBSS43 | B18 | Coding | >SRR8088272_GL0062831 |
| BBa_253RKAEK | B19 | Coding | >SRR10912790_GL0087456 |
| BBa_25IDFGN2 | B20 | Coding | >SRR2637690_GL1217358 |
New improved parts
14 improved enzyme coding sequences
New Improved PET-Degrading Enzyme Parts Catalog
| Part Name | Name/Short Description | Part Type |
|---|---|---|
| BBa_25KJ70I6 | V208L | Coding |
| BBa_258QGPDE | D248M | Coding |
| BBa_25ZD50KL | D248K | Coding |
| BBa_25FZ65J | D234K | Coding |
| BBa_25C6EYZY | D234S | Coding |
| BBa_255GPXNI | D242R | Coding |
| BBa_25Y2H3J4 | D213R | Coding |
| BBa_25PL3L0O | A121C-A143C | Coding |
| BBa_25LSL3DH | dsPET05 mutant D2(I150A-L199A) | Coding |
| BBa_256ZHBKO | dsPET05 mutant(D5 I150A-S203T) | Coding |
| BBa_25QH8A7I | dsPET05 mutant D14(I150L-L199A) | Coding |
| BBa_253OWT5E | dsPET05 mutant D17(I150L-S203T) | Coding |
| BBa_258YTIHX | dsPET05 mutant D30(I176A-S200A) | Coding |
| BBa_25YTAJ4Y | dsPET05 mutant D36(I176A-S203T) | Coding |