Protocols-Wetlab

Bacterial culture

Antibiotic stock
LB medium
M9 medium
TSB medium
Refolding buffer
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Molecular biology

PCR (Q5)
PCR (KOD One)
PCR cleanup
Gibson assembly
DNA phospholigation
Electrophoresis
Plasmid extraction (miniprep)
gRNA extraction
Competent cells preparation using CaCl2
Heat shock (CaCl2 method)
Competent cells preparation using Rubidium
Heat shock (Rubidium method)
Restriction enzyme digestion
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FluorMango

FluorMango synthesis
FluorMango sensitivity assay
FluorMango specificity assay
FluorMango assay on monofluorinated substrate
FluorMango microfluidic test
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Others

Toxicity assays
PFOS degradation
PFOA adsorption on beads
Release/ Stability of encapsulated bacteria
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