In order to systematically address the limitations of traditional allergen immunotherapy (AIT), such as long treatment duration, safety risks, and complex administration methods. We modified common allergens (pollen allergens, dust mite allergens, and seafood allergens) to obtain hypoallergenic derivatives, in order to minimize the risk of allergic reactions while maintaining T-cell reactivity. During this process, we also constructed both hypoallergenic derivatives and natural allergens. After successful verification, we combined the hypoallergenic derivatives and connected self-assembling peptides (EFK8) to promote oligomerization, enhance stability, prolong the serum half-life, and achieve targeted effects against two allergens in a single molecule.
We have documented these parts and conducted comprehensive tests, including plasmid construction, expression strain construction, protein expression and purification, and functional validation. Our goal is to obtain a safer, more effective, and widely applicable immunotherapy strategy.
Table 1 Part collection in this project.
|
Part number |
Part name |
Part type I |
Part type II |
|
mPhl p1 |
Basic part |
New part |
|
|
mBet v1 |
Basic part |
New part |
|
|
mDer p1 |
Basic part |
New part |
|
|
mDer p2 |
Basic part |
New part |
|
|
mMet e1 |
Basic part |
New part |
|
|
mCyp c1 |
Basic part |
New part |
|
|
rPhl p1 |
Basic part |
New part |
|
|
rBet v1 |
Basic part |
New part |
|
|
rDer p2 |
Basic part |
New part |
|
|
rMet e1 |
Basic part |
New part |
|
|
rCyp c1 |
Basic part |
New part |
|
|
pET32a-backbone |
Basic part |
New part |
|
|
TrxA |
Basic part |
Existing part |
|
|
F peptide （EFK8） |
Basic part |
New part |
|
|
PT linker |
Basic part |
New part |
|
|
(GS)4 linker |
Basic part |
New part |
|
|
BBa_K4587201 |
His-tag |
Basic part |
Existing part |
|
BBa_K2277033 |
EGFP |
Basic part |
Existing part |
|
pET32a-mPhl p1-His tag |
Composite part |
New part |
|
|
pET32a-mBet v1-His tag |
Composite part |
New part |
|
|
pET32a-mDer p1-His tag |
Composite part |
New part |
|
|
pET32a-mDer p2-His tag |
Composite part |
New part |
|
|
pET32a-mMet e1-His tag |
Composite part |
New part |
|
|
pET32a-mCyp c1-His tag |
Composite part |
New part |
|
|
pET32a-rphl p1-His tag |
Composite part |
New part |
|
|
pET32a-rBet v1-His tag |
Composite part |
New part |
|
|
pET32a-rDer p2-His tag |
Composite part |
New part |
|
|
pET32a-rMet e1-His tag |
Composite part |
New part |
|
|
pET32a-rCyp c1-His tag |
Composite part |
New part |
|
|
pET32a-EFK8-PT-mPhl p1-GS-mBet v1-His tag |
Composite part |
New part |
|
|
pET32a-EFK8-PT-mDer p1-GS-mDer p2-His tag |
Composite part |
New part |
|
|
pET32a-EFK8-PT-mMet e1-GS-mCyp c1-His tag |
Composite part |
New part |
|
|
pET32a-EFK8-PT-EGFP-His tag |
Composite part |
New part |
