Parts

Overview

This project comprises 15 parts that together establish a reusable DBTL workflow for broad-spectrum AMP engineering in E. coli. The initial SUMO-tag expression suffered from low yield and host toxicity; therefore, we retained the SUMO tag and introduced an anionic-fusion strategy to construct SUMO–EELDNALN–EDPNG–Ulink-AMP and its variants, improving solubility and reducing toxicity.

MIC and agar-diffusion assays confirmed that all variants inhibited both E. coli and B. subtilis, with AMP-TB2 (G40A) showing the strongest activity. Molecular docking further demonstrated stable binding of TB2 to key targets from Staphylococcus aureus, Campylobacter, and Salmonella, while temperature-stability tests suggested better activity retention against Gram-positive bacteria.

Together, these parts constitute a standardized module that integrates rational design, expression optimization, and functional validation—providing a clear and reproducible framework for future peptide-based antibacterial system development.

Parts list