Endolysin platform protocols

Creation of chemically competent cells

Transformation of the chemically competent cells

Colony PCR insert amplification

Plasmid DNA purification

Insert amplification: Q5 HF PCR

Restriction enzyme digestion

QIAGEN PCR purification

Gel extraction of plasmid backbone

Golden Gate Assembly

Ligation

Bead protein purification

Bactericidal Effect

Biofilm destruction protocols

IPTG/X-Gal agar plates

LB, Top Agar, and SOC media

Electrocompetent E. coli K12 ER2738

E. coli K12 ER2738 glycerol stock

Propagating plaques and isolating phages

Phage lysis and DNA purification

Biofilm assay

Other protocols

Buffers, LB Medium, Agarose Gel and Antibiotics

IDT Gene Preparation

cPCR and PCR

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