To obtain reliable quantified results for the purity and concentration of the eluted DNA isolated through maxi prep we performed the following assays. First, to determine DNA concentration we measured absorbance at 260 nm using the JENWAY Genova Plus UV-Vis spectrophotometer, which indicated 99.4 ng/μL. The DNA concentration of 99.4 ng/μL shows a sufficient yield, which was determined acceptable for the continuation of our investigation. We diluted at 1:10 with dH₂O and using the same apparatus we measured absorbance at 260/280 nm to determine DNA purity. We got a ratio of 1.39. The resulting ratio indicates that there is a likely presence of contaminants that absorb more significantly at 280 nm.

To visualize our isolation product of plasmid DNA we performed a Southern blot in agarose gel. Using gel electrophoresis, we were able to observe a band that indicates the presence of DNA in the sample. Based on the position of the band in the gel and its density, it is highly likely that the DNA of the sample is approximately 10kb and thus could plausibly correspond to our plasmid (~11kb). However, lack of 10kb ladders in the lab prevents us from making absolute conclusions.