Part Collection
Our team contributed significantly to the iGEM Registry by uploading 13 new parts.
These parts include 5 new AChE coding variants as basic parts, including five point mutants and corresponding plasmid constructs as composite parts. We systematically characterized each mutant of the wild-type AChE, demonstrating that several mutants outperform the wild-type enzyme. Based on these parts, we created a Part Collection that provides optimized enzymes for constructing AChE-based biosensors, including the pesticide residue detection strips. This effort not only enhances the Registry’s diversity but also provides reliable, stable tools for the rapid detection of pesticide residues.
We nominated BBa_25RB3BMI, BBa_25RAEM2G, BBa_2553XFCD, BBa_25Y5WN3J, BBa_25OIMLTT and BBa_253PQ99W for the Best Part Collection award.
The detailed documentation of these parts is available at:
https://registry.igem.org/collections/2e4fae2b-aa49-461c-a82f-78a9a7f8c7b5
All parts submitted:
These parts include 5 new AChE coding variants as basic parts, including five point mutants and corresponding plasmid constructs as composite parts. We systematically characterized each mutant of the wild-type AChE, demonstrating that several mutants outperform the wild-type enzyme. Based on these parts, we created a Part Collection that provides optimized enzymes for constructing AChE-based biosensors, including the pesticide residue detection strips. This effort not only enhances the Registry’s diversity but also provides reliable, stable tools for the rapid detection of pesticide residues.
We nominated BBa_25RB3BMI, BBa_25RAEM2G, BBa_2553XFCD, BBa_25Y5WN3J, BBa_25OIMLTT and BBa_253PQ99W for the Best Part Collection award.
The detailed documentation of these parts is available at:
https://registry.igem.org/collections/2e4fae2b-aa49-461c-a82f-78a9a7f8c7b5
All parts submitted:
| Biobricks | Name | Type I | Type II | Description |
|---|---|---|---|---|
| BBa_25BUF4NW | AChE_WT | Basic | coding | Wild-type (unoptimized)AChE |
| BBa_25RB3BMI | AChE_D203A | Basic | coding | mutation at the 203 site |
| BBa_25RAEM2G | AChE_Y355A | Basic | coding | mutation at the 355 site |
| BBa_2553XFCD | AChE_Y396A | Basic | coding | mutation at the 396 site |
| BBa_25Y5WN3J | AChE_N404A | Basic | coding | mutation at the 404 site |
| BBa_25OIMLTT | AChE_E466A | Basic | coding | mutation at the 466 site |
| BBa_253PQ99W | pET-32a backbone | Basic | Plasmid backbone | optimized to be RFC1000 compatible |
| BBa_25WQYNUR | AChE(WT)_pET32a | Composite | Plasmid | Wild type expression vector |
| BBa_2517HF0V | AChE(D203A)_pET32a | Composite | Plasmid | Mutant D203A cloned to pET_32a vector |
| BBa_25XC893G | AChE(Y355A)_pET32a | Composite | Plasmid | Mutant Y355A cloned to pET_32a vector |
| BBa_25VBBVSV | AChE(Y396A)_pET32a | Composite | Plasmid | Mutant Y396Acloned to pET_32a vector |
| BBa_25R918KH | AChE(N403A)_pET32a | Composite | Plasmid | Mutant N403A cloned to pET_32a vector |
| BBa_252SF7IG | AChE(E466A)_pET32a | Composite | Plasmid | Mutant E466A cloned to pET_32a vector |
Designing Improved Test Strips
Based on the colloidal gold technology, we have proposed an improved test strip design. This new design provides real life application for our part collection, enabling rapid on-site detection of pesticide residues in food and environmental samples. The design emphasizes simplicity, economy and reliability, reflecting our focus on practical applications.
Test Strip Design
Software Design
To complement our laboratory experiments, we developed software to quantify the results of our test strips. This tool can automatically measure color changes and provides highly accurate pesticide residues, reducing human errors and improving repeatability.
Currently, this software is still under development, but you can view our design on the Software
Currently, this software is still under development, but you can view our design on the Software
Science Communication and Outreach
Beyond laboratory work, our team actively contributed to science communication and education. We collaborated with CJUH-JLU-China to write the “crushing the myths of synthetic biology” brochure. We also promoted the work of female scientists in STEM, highlighting their contributions and inspiring younger generations.
