Parts
We nominated BBa_25RB3BMI, BBa_25RAEM2G, BBa_2553XFCD, BBa_25Y5WN3J, BBa_25OIMLTT and BBa_253PQ99W for the Best Part Collection award.
The detailed documentation of these parts is available at:
https://registry.igem.org/collections/2e4fae2b-aa49-461c-a82f-78a9a7f8c7b5
All parts submitted:
The detailed documentation of these parts is available at:
https://registry.igem.org/collections/2e4fae2b-aa49-461c-a82f-78a9a7f8c7b5
All parts submitted:
| Biobricks | Name | Type I | Type II | Description |
|---|---|---|---|---|
| BBa_25BUF4NW | AChE_WT | Basic | coding | Wild-type (unoptimized)AChE |
| BBa_25RB3BMI | AChE_D203A | Basic | coding | mutation at the 203 site |
| BBa_25RAEM2G | AChE_Y355A | Basic | coding | mutation at the 355 site |
| BBa_2553XFCD | AChE_Y396A | Basic | coding | mutation at the 396 site |
| BBa_25Y5WN3J | AChE_N404A | Basic | coding | mutation at the 404 site |
| BBa_25OIMLTT | AChE_E466A | Basic | coding | mutation at the 466 site |
| BBa_253PQ99W | pET-32a backbone | Basic | Plasmid backbone | optimized to be RFC1000 compatible |
| BBa_25WQYNUR | AChE(WT)_pET32a | Composite | Plasmid | Wild type expression vector |
| BBa_2517HF0V | AChE(D203A)_pET32a | Composite | Plasmid | Mutant D203A cloned to pET_32a vector |
| BBa_25XC893G | AChE(Y355A)_pET32a | Composite | Plasmid | Mutant Y355A cloned to pET_32a vector |
| BBa_25VBBVSV | AChE(Y396A)_pET32a | Composite | Plasmid | Mutant Y396Acloned to pET_32a vector |
| BBa_25R918KH | AChE(N403A)_pET32a | Composite | Plasmid | Mutant N403A cloned to pET_32a vector |
| BBa_252SF7IG | AChE(E466A)_pET32a | Composite | Plasmid | Mutant E466A cloned to pET_32a vector |