We are honored to have our project experiment in the laboratory space in the Institute of Biophysics, Chinese Academy of Sciences. Before and during the experiment, we have received extensive training on laboratory safety. There are several regulations during different experimental procedures. Before the experiment began, when we entered the laboratory, we had to put on the lab coats, gloves, and masks to protect ourselves and protect experimental samples and devices to reduce error. In the experiment, we extract plasmids using Escherichia coli. Some strains of E. coli can cause severe foodborne illnesses, like diarrhea and abdominal cramps. Hence, after completing the experiment, it's also important to take off protective clothing to prevent biological pollution.

In the process of experimentation, we use a biological cabinet when we deal with some samples that require high precision or are harmful to the human body. The cabinet could draw the air inside the cabinet outwards, maintaining a negative pressure inside the cabinet. It achieves this by using a vertical airflow to protect the workers.

Before using the biological cabinet, we first wipe the working platform inside the safety cabinet thoroughly with 70% alcohol or other disinfectants.

After putting the sample into the cabinet, close the glass door and leave a little space for operation. Then, turn on the power switch, and if necessary, turn on the ultraviolet lamp to disinfect the surface of the experimental items. After the process is completed, set the device to the safe cabinet operation mode, open the glass door, and allow the machine to operate normally. After the equipment has completed the cleaning process and is running stably, it can be put into use. After completing the work and removing the waste, we clean the platform. Maintain the air circulation for a period of time to allow the pollution in the work area to be absent.

On the table in the laboratory, we have a medical waste garbage can. We put the Pipette tip and liquid waste into the garbage bag after use. When the experiment is complete, the garbage will be disposed of.

When conducting PCR, certain regulations need to be followed to ensure the accuracy and reliability of the experiment. The experimental procedures should be carried out in a single direction to prevent cross-infection. In the PCR laboratory, there are four areas. Between them are two doors that could measure air pressure. In this condition, we should close the first door and then open another door to keep the laboratory clean. After the analysis is completed, the reaction tubes should be placed in the local waste bags. Then, we will take the waste out of the laboratory and dispose.

Our experiments involved utilization of several kinds of pesticide selling on the market. To ensure the safety of our pesticide testing process, we have established a strict set of protective measures. Firstly, we followed the laboratory biosafety guidelines and used appropriate personal protective equipment to minimize direct contact with pesticide samples. All pesticide handling was conducted in fume hoods or well-ventilated areas to prevent accidental inhalation of volatile compounds. In terms of waste management, all pesticide residues and contaminated materials are collected separately and handled in accordance with the regulations for hazardous chemicals to ensure that harmful substances do not leak into the environment. At the same time, our experimental design strictly controlled the amount of pesticide solution used in each test, reducing exposure risks.
The types of pesticide we used have been updated in the final safety form, which is approved by the iGEM HQ.

We strictly adhered to the requirements for HP content displayed in the iGEM Human Practices guidelines. All the interviews were conducted with the consent of the interviewees, and all the recordings and notes were made known to the interviewees. Informed consents were obtained both oral or written.

Informed consent (translated version):
By proceeding with this interview, you are agreeing to participate in a research project for BAID-China 2025 in the iGEM (International Genetic Engineering Machine) competition. This interview will be used for iGEM only. Participation is completely voluntary, and you may end this interview at any time without penalty. There are no risks to your personal information. Your responses will contribute to research into improving synthetic biology intercommunication. In case you have a question, you can contact [The team member responsible for this interview] at [his/her email address & his/her Wechat].