Overview
Team BIT registered 8 basic parts and 7 new composite parts.
Basic Parts
Our basic parts include fundamental biological components designed for targeted drug delivery and cancer cell recognition.
| Part Code | Part Name | Function & Description | Length |
|---|---|---|---|
| BBa_25VDY74P | Negative Charge Sequence | The negative-charge sequence utilized to reduce the positive surface potential of the fusion protein. | 27 bp |
| BBa_2541L2J9 | MMP9-Specific Cleavable Sensitive Peptide | Sensitive Peptide can be specifically cleaved by MMP9 and is used for targeting tumor cells. | 18 bp |
| BBa_25PL3I9A | BR Ⅱ | The cell-penetrating peptide employed to carry drugs into cells. | 51 bp |
| BBa_254B51K9 | IL-24 | Protein-based drugs used to kill cancer cells. | 465 bp |
| BBa_25Y42G2Q | SOS-box | The oxidative damage-activated promoter is used to sense DNA damage and initiate downstream gene expression. | 47 bp |
| BBa_25W6QRPL | LuxI | Encoding AHL synthase which promotes the synthesis of the signaling molecule AHL. | 579 bp |
| BBa_25R3FP1Y | Plux | Quorum-sensing promoter, which can be activated by the AHL-LuxR homodimer to initiate the expression of downstream genes. | 35 bp |
| BBa_25OAIB4Z | LuxR | Encoding LuxR protein, which forms a complex with AHL to activate the promoter Plux. | 770 bp |
Composite Parts
Our composite parts combine multiple basic components to create complex functional modules for tumor targeting and drug delivery.
| Part Code | Part Name | Function & Description | Length |
|---|---|---|---|
| BBa_25D22FIV | TRACER | Drug delivery system, used to carry protein drugs and target cancer cells. | 112 bp |
| BBa_25VAOI8Q | TRACER-IL-24 | Drug delivery system carrying IL-24, used to carry drugs and target and kill cancer cells. | 611 bp |
| BBa_253FAAIE | SOS-LuxI | Environment-responsive module used to sense the oxidative damage environment and convert it into the intracellular signal AHL. | 655 bp |
| BBa_25TOL6JM | J23100-LuxR | Constitutive expression of LuxR used to bind with the signaling molecule AHL and induce downstream promoter. | 834 bp |
| BBa_255T4KD9 | Plux-SRRz | Lysis system used to respond to upstream signals and initiate the suicidal lysis program. | 1714 bp |
| BBa_25RV0S02 | SOS-LuxI-J23100-LuxR | Sensing the tumor microenvironment with high oxidative damage and converting it into AHL signaling molecules to induce downstream gene expression. | 1703 bp | BBa_25M0U6S3 | Oxidative Damage-Response Lysis Module | The environment response-lysis release module is our entire tumor-recognition and drug-release module: The tumor microenvironment exhibits high oxidative damage due to massive infiltration of inflammatory cells and mitochondrial dysfunction, inducing DNA damage. The SOS-box is induced by DNA damage, initiating downstream LuxI gene expression. The LuxI gene expresses AHL synthase, which catalyzes the synthesis of AHL signaling molecules. Meanwhile, the constitutive promoter PJ23100 promotes downstream LuxR gene to express LuxR protein. Two molecules of AHL and two molecules of LuxR protein form a homodimer, inducing the downstream Plux promoter to initiate the expression of lysis gene SRRz, and cell lysis releases drugs. | 3513 bp |
Reference Parts
Standard biological parts from the iGEM registry used in our constructs.
| Part Code | Part Name | Function & Description | Length |
|---|---|---|---|
| BBa_B0030 | RBS.1 (strong) | Strong RBS Modified from R. Weiss. | 15 bp |
| BBa_J23100 | Constitutive Promoter Family Member | Constitutive promoter used for constitutive expression of the LuxR gene. | 35 bp |
| BBa_K4818021 | Lambda t0 Terminator | A specific transcription terminator sequence in the genome of the lambda phage. | 95 bp |
| BBa_C0261 | LuxI | The activator protein LuxI in Lux gene regulatory network. | 661 bp |
| BBa_C0062 | LuxR | The activator protein luxR in lux gene regulatory network. | 781 bp |