Software

Our Epitope predictor software provides future teams an either way to run in silico tests on the effectiveness of enzymes meant to break up antigens to prevent an immune response. The predictor relies entirely on user input data, meaning that it will work for any protein, enzymes and any amino acid sequences the user wishes to test.

In any situation where a protein is digested with an enzyme and the fragments are bound to another protein motif, this code can be used to model that response in silico. Quickly, easily and on as many sequences as your computer can keep up with.

Built in R, an open source language based around statistics and using standard non-proprietary file types such as CSVs and FASTA files ensures that the code will be easily accessible to everyone and can not only be used by others but can be modified to meet their unique needs. We hope this will help future teams and researchers by saving them time and resources in exploring enzyme selection and effects in their experiments.

For more on our Software please consult our Software page!

Parts

The addition of the pair of proteins P61 (BBa_25JAUHW7) and P64 (BBa_259UYKNX) constitutes a valuable contribution to the synthetic biology community, expanding the toolkit available for modular biological design. Our DNA sequence allows future teams to express the peptide capping proteins.

Having combined all the necessary components for bacterial protein expression with three different secretion tags, it is very easy to implement the sequence into a plasmid via Golden Gate assembly, allowing future teams to build on them. Furthermore, the addition of the P61 and P64 proteins could inspire new project ideas such as novel bioindicators, or allow expansion on pre-existing ones.

For more information on the Peptide Caps please consult our Parts page!