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Experiments

To ensure that our project is transparent, reproducible, and well-documented, we maintained a structured workflow for recording our experiments. Every day, all laboratory work was carefully noted in our Notebook. For each experiment, we documented the objective, the team members involved, and the protocols and kits used. This systematic approach allowed us not only to track our progress but also to reflect on our results and improve our methods over time.

In addition to our physical and digital Notebook, we also used a dedicated eLab portal. This platform served as a central hub where all experimental data, updated protocols, and analyzed results were uploaded and continuously updated. The eLab ensured that every member of the team had access to the latest information and that no details were lost in the fast-paced environment of the laboratory.

By combining daily entries in the Notebook with the structured digital archive in the eLab, we created a comprehensive record of our project. This dual system provided clarity, traceability, and accessibility, which are essential both for the internal workflow of the team and for sharing our work with the wider iGEM community.

Overview

Specific parts

I_2025_S000_Creation_of_general_parts

I_2025_S001_E003 Assembly, Transformation and Verification of mCherry L1 - Kaempferol Sensor

I_2025_S002_E001_Creation of L1 Kaempferol Pathway Parts

I_2025_S003_E002_Creation of L2 Vanillin Pathway Parts

I_2025_S004_E002_Creation of Vanillin Sensor L2s

Protocols

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