Parts

Number	Short Desc / Name	Registry Part Name	Type	Specification	Function
1	MCF_Pos_AB	BBa_25USX7KG	Basic Part	Plasmid Backbone	MoCloFlex Position Vector AB from https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2019.00271/full
2	MCF_Pos_CD	BBa_253ROOBB	Basic Part	Plasmid Backbone	MoCloFlex Position Vector CD from https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2019.00271/full
3	MCF_Pos_EF	BBa_25LZZPKF	Basic Part	Plasmid Backbone	MoCloFlex Position Vector EF from https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2019.00271/full
4	MCF_Pos_GH	BBa_25T60D9F	Basic Part	Plasmid Backbone	MoCloFlex Position Vector GH from https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2019.00271/full
5	MCF_Pos_IJ	BBa_255UK1EW	Basic Part	Plasmid Backbone	MoCloFlex Position Vector IJ from https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2019.00271/full
6	L0_entry_Vector	BBa_25W08SJQ	Basic Part	Plasmid Backbone	Plasmid backbone with drop-out GFP
7	J23100	BBa_J23100	Basic Part	Promoter	The J23100 Anderson promoter is a strong, constitutive promoter from the iGEM Anderson library, widely used as a high-expression benchmark in E. coli.
8	J23114	BBa_J23114	Basic Part	Promoter	The J23114 Anderson promoter is a constitutive promoter from the iGEM Anderson library with lower strength than J23100, yielding modest, steady transcription in E. coli
9	pqdoI + RBS	BBa_25BPYX9D	Basic Part	Promoter	PqdoI is the QdoR-regulated promoter from the qdoI operon (e.g., Bacillus), repressed by QdoR and activated by flavonols like kaempferol (and quercetin). It’s used as a small-molecule–inducible promoter to drive expression in response to kaempferol.
10	pVan	BBa_2519OUCA	Basic Part	Promoter	pVan is the vanillic acid–inducible output promoter in the Marionette E. coli strains, activated by the VanR regulator to give high dynamic range with low leak and minimal cross-talk. It’s used to drive gene expression specifically in response to vanillic acid
11	pTet	BBa_25JNK0I4	Basic Part	Promoter	pTet is the TetR-repressible promoter that’s induced by tetracycline/anhydrotetracycline (aTc); TetR blocks transcription until inducer binding relieves repression. In Marionette E. coli, it serves as an output promoter with low leak and strong induction in response to aTc.
12	Plac	BBa_25OH37D3	Basic Part	Promoter	pLac is the classic LacI-repressible, IPTG/lactose-inducible σ⁷⁰ promoter from E. coli; LacI blocks transcription at the operator until inducer binding relieves repression.
13	pL_lac0_1	BBa_J428041	Basic Part	Promoter	Strong LacI and Lambda-CI repressible promoter. Promoter was originally derived by hybridizing the pL promoter from lambda phage with lacO sites.
14	B0034	BBa_J428038	Basic Part	RBS	BBa_B0034 for MoClo Level 0 with 7bp scar/spacer
15	sfGFP	BBa_25F7XNIC	Basic Part	CDS	sfGFP (superfolder GFP) is a monomeric green fluorescent protein engineered with folding mutations for high brightness, rapid maturation, and robust folding even at 37 °C and in difficult fusions. It’s widely used as a reporter or fusion tag, with excitation ~488 nm and emission ~510 nm.
16	mCherry	BBa_2554G8BU	Basic Part	CDS	mCherry is a monomeric red fluorescent protein (Ex/Em ~587/610 nm) with fast maturation and good photostability, widely used as a reporter and fusion tag.
17	mTagBFP	BBa_K592100	Basic Part	CDS	This part codes for the bright blue fluorescent protein mTagBFP. mTagBFP is a monomeric protein with a narrow fluorescence emission spectrum with a maximum at 456 nm. It has a tyrosine-based chromophore, giving it substantially higher brightness, faster chromophore maturation and higher pH stability than blue fluorescent proteins with a histidine in the chromophore.
18	mScarlett	BBa_25IT4NB1	Basic Part	CDS	mScarletis a bright, monomeric red fluorescent protein with fast maturation and high quantum yield (Ex/Em ~569/594 nm), ideal as a reporter or fusion tag.
19	Qdor	BBa_252CF98L	Basic Part	CDS	QdoR is a MarR-family transcriptional repressor that binds the pqdoI operator and blocks transcription; flavonols like kaempferol (and quercetin) reduce its DNA affinity to induce expression
20	VanR	BBa_25G4NKL	Basic Part	CDS	VanR is the vanillic acid–responsive transcription factor that controls the pVan promoter; binding vanillate modulates its DNA affinity to turn on target gene expression with low leak and high dynamic range.
21	ECH	BBa_25R9DFSZ	Basic Part	CDS	ECH (enoyl-CoA hydratase/aldolase) catalyzes the key step of the CoA-dependent ferulate pathway, converting feruloyl-CoA to vanillin (via a β-hydroxy intermediate) and acetyl-CoA.
22	FCS	BBa_25YBLOQG	Basic Part	CDS	Fcs (feruloyl-CoA synthetase) activates ferulate to feruloyl-CoA using ATP and CoA, forming AMP and PPi. It’s the entry enzyme of the CoA-dependent ferulate→vanillin pathway, feeding substrates to ECH and then Vdh.
23	LacI-AM	BBa_2590H0LP	Basic Part	CDS	LacI_AM is an engineered LacI repressor used in Marionette E. coli that tightly represses pLac with low leak and strong dynamic range, and is specifically inducible by IPTG. It’s optimized for orthogonality and consistent performance across the Marionette sensor set.
24	TetR-AM	BBa_25FFU03N	Basic Part	CDS	TetR_AM is an engineered TetR repressor used in Marionette E. coli that tightly controls pTet, showing very low leak and high dynamic range. It’s specifically inducible by tetracycline/anhydrotetracycline (aTc) and optimized for orthogonality across the sensor set.
25	Cufls	BBa_25Y70HTS	Basic Part	CDS	cuFLS (flavonol synthase) is an Fe(II)/2-oxoglutarate–dependent dioxygenase that converts dihydrokaempferol into kaempferol (and related flavonols). It’s commonly used in engineered pathways to boost kaempferol production.
26	cis3h	BBa_25XPKX63	Basic Part	CDS	cisF3H (flavanone 3-hydroxylase, likely from Citrus sinensis) is an Fe(II)/2-oxoglutarate–dependent dioxygenase that converts flavanones (e.g., naringenin) to dihydroflavonols (e.g., dihydrokaempferol), a key upstream step in kaempferol biosynthesis.
27	EF_J23100_cufls+cis3h	BBa_25Q96FFK	Composite part	Composite	This construct places cisF3H and cuFLS in a single operon under the strong constitutive J23100 promoter. cisF3H converts flavanones (e.g., naringenin) to dihydroflavonols, and cuFLS then produces kaempferol
28	MCF_Pos_AB + p15a	BBa_25BXR8T0	Composite part	Plasmid Backbone	Adjusted MoCloFlex Position AB Plasmid backbone with p15a Ori
29	MCF_AB_no_Ori	BBa_25PJXD4U	Basic Part	Plasmid Backbone	MocloFlex Position AB Plasmid backbone with cutting sites to swap out Ori
30	BC_Linker	BBa_25W7GVGY	Basic Part	Linker	Part that links MoCloFlex Position Vectors AB with CD
31	CD_SmR	BBa_259MEHJ6	Composite part	Antibiotic Resistance	Spectinomycin Resistance cassette on MocloFlex Position CD Plasmid Backbone
32	DF_Linker	BBa_25JMI1VR	Basic Part	Linker	Part that links MoCloFlex Position Vectors CD with EF but rotates the direction of EF
33	EF_pqdoI_sfGFP	BBa_25ELZQOF	Composite part	Composite	sfGFP inducible by Kaempferol
34	EG_Linker	BBa_25QMW3D4	Basic Part	Composite	Part that links MoCloFlex Position Vectors EF with GH but rotates the direction of EF
35	GH_J23114_Qdor	BBa_257RMT91	Composite part	Composite	Qdor moderately expressed
36	HI_Linker	BBa_253EDVDS	Basic Part	Linker	Part that links MoCloFlex Position Vectors GH with IJ
37	IJ_J23100_mCherry	BBa_25UVAOFA	Composite part	Composite	Strongly expresses mCherry
38	JA_Linker	BBa_252PH30R	Basic Part	Linker	Part that links MoCloFlex Position Vectors IJ with AB
39	EF_pVan_sfGFP	BBa_25ZD8EEI	Composite part	Composite	sfGFP inducible by Vanillic acid
40	GH_J23114_VanR	BBa_25VGOVPE	Composite part	Composite	Moderately expresses VanR
41	EF_pvan_mTagBFP	BBa_25V6JOSO	Composite part	Composite	mTagBFP inducible by Vanillin acid
42	B0015	BBa_J428092	Basic Part	Terminator	B0015 is a strong, standard BioBrick double terminator (rrnB T1 + T7Te) that provides highly efficient transcriptional termination and minimizes read-through. It’s widely used to insulate transcription units and stabilize expression.
43	MC L0 PcymRC	BBa_25143IE9	Basic Part	Promoter	Robust, tunable induction with cuminic acid. Useful for combinatorial tuning with minimal cross-talk in multi-inducer setups.
44	MC L0 pPhlf	BBa_2585P3D3	Basic Part	Promoter	Tight, orthogonal induction with 2,4-diacetylphloroglucinol (DAPG). Adds an independent expression “channel” for multiplex control.
45	MC L0 pttg	BBa_251N6Q25	Basic Part	Promoter	Flavonoid-responsive channel suited to metabolic engineering or biosynthesis demos; strong induction with controlled leak.
46	MC L0 p3B5B	BBa_25LGVXAV	Basic Part	Promoter	An additional benzene-derivative–responsive channel (3,4-dihydroxybenzoic acid), handy when many independent knobs are needed.
47	MC L0 pSalTCC	BBa_25ZYRA0N	Basic Part	Promoter	Salicylate-responsive expression with low background; good companion channel in multiplex designs.
48	MC L0 pCin	BBa_25S6UI57	Basic Part	Promoter	A quorum-sensing–style promoter responsive to long-chain AHL (3-hydroxytetradecanoyl-HSL); useful for cell–cell communication modules.
49	AB_pTet_ECH	BBa_25RULR65	Composite part	Composite	This construct drives ECH (enoyl-CoA hydratase/aldolase) from the pTet promoter, enabling aTc/tetracycline-inducible expression. In TetR-controlled systems (e.g., Marionette), it stays tightly repressed with low leak until induction, allowing timed flux through the ferulate→vanillin pathway
50	CD_plac_FCS	BBa_25T9RABL	Composite part	Composite	This construct expresses Fcs (feruloyl-CoA synthetase) from the pLac promoter, giving IPTG-tunable induction under LacI control. Inducing boosts conversion of ferulate → feruloyl-CoA, feeding the upstream step of the vanillin pathway
51	EF_J23100_LacI	BBa_25LQMIMM	Composite part	Composite	This construct places LacI under the strong constitutive J23100 promoter in the MoCloFlex Position Vector EF, so it continuously supplies LacI to repress pLac modules.
52	GH_J23100_TetR	BBa_255PO8JP	Composite part	Composite	This construct places TetR under the strong constitutive J23100 promoter in the MoCloFlex Position Vector GH, providing continuous TetR to repress pTet modules.
53	CD_GmR	BBa_256OYJG	Composite part	Antibiotic Resistance	Gentamicin Resistance cassette on MocloFlex Position CD Plasmid Backbone
54	Destination Vector XY	BBa_25BPPV6F	Basic Part	Plasmid Backbone	MoCloFlex Destination Vector XY carries the X–Y destination motifs (BbsI) flanking a ccdB/lacZ dropout, so assemblies from position vectors are delivered via end-linkers into the final plasmid—and it supports iterative rounds of cloning while staying MoClo-compatible.
55	CA Linker	BBa_25AU5UVU	Basic Part	Linker	Part that links MoCloFlex Position Vectors AB with CD
56	XB Linker	BBa_25VJTOV7	Basic Part	Linker	Part that links MoCloFlex Destination Vector XY with Position Vector AB
57	HY Linker	BBa_25A27SRE	Basic Part	Linker	Part that links MoCloFlex Destination Vector XY with Position Vector GH
58	EY Linker	BBa_25IQE7EW	Basic Part	Linker	Part that links MoCloFlex Destination Vector XY with Position Vector EF
59	IJ_plac01_cufls	BBa_2544HX34	Composite part	Composite	This construct expresses cuFLS (flavonol synthase; an Fe(II)/2-oxoglutarate dioxygenase that converts dihydrokaempferol → kaempferol) from the IPTG-inducible pLac01 promoter in the MoCloFlex Position Vector IJ
60	CD_ptet_cisf3h	BBa_25693ONF	Composite part	Composite	his construct expresses cisF3H (flavanone 3-hydroxylase; Fe(II)/2-oxoglutarate dioxygenase converting flavanones → dihydroflavonols) from the pTet promoter in the MoCloFlex Position Vector CD
61	Kaempferol Sensor L2	BBa_25D2WDUC	Composite part	Composite	This MoCloFlex L2 construct sits on a p15A backbone with spectinomycin resistance and comprises three modules: (1) pqdoI→sfGFP as a kaempferol-inducible reporter TU, (2) QdoR driven by J23114 to repress pqdoI with low leak until inducer is present, and (3) mCherry under strong J23100 as a constitutive normalization/visual marker. Kaempferol specifically lifts QdoR repression to induce GFP, while mCherry provides a constant reference signal for ratiometric readouts
62	Vanillin Sensor L2	BBa_25327WS6	Composite part	Composite	This MoCloFlex L2 construct contains three modules on a p15A, spectinomycin-resistant backbone: (1) pVan→sfGFP as an inducible reporter TU, (2) VanR driven by J23114 for moderate, low-leak control of pVan, and (3) mCherry under strong J23100 as a constitutive normalization/visual marker. Vanillic acid (vanillate) specifically induces GFP via VanR–pVan, while mCherry provides a constant reference signal for ratiometric readouts
63	Kaempferol Pathway L2	BBa_2584WSJ4	Composite part	Composite	This MoCloFlex L2 construct contains two inducible pathway TUs—pLac→cuFLS (IPTG-tunable conversion of dihydrokaempferol → kaempferol) and pTet→cisF3H (aTc/tetracycline-inducible hydroxylation of flavanones → dihydroflavonols)—plus two regulator TUs, J23114→LacI and J23114→TetR, to keep pLac and pTet tightly repressed at baseline
64	Vanillin Pathway XY L2	BBa_253AIYTG	Composite part	Composite	This MoCloFlex L2 construct is assembled on the Destination Vector XY backbone and comprises four TUs: pTet→ECH to enable aTc/tetracycline-inducible flux through the ferulate→vanillin pathway, pLac→Fcs for IPTG-tunable activation of ferulate to feruloyl-CoA, J23114→TetR to keep pTet tightly repressed until induction, and J23114→LacI to repress pLac with low leak. Together, LacI/TetR provide moderate, constitutive repressor levels for clean ON/OFF control, while the XY destination backbone keeps the assembly MoCloFlex-compatible for iterative builds