Protocol list:
- Preparation of culture medium
- Shaking the bacterial culture
- Extract plasmids from bacterial cells
- PCR amplification
- Agarose Gel electrophoresis
- Gel DNA extraction
- PCR products purify extraction
- E. coli transformation
- Solid plate preparation and spread methods
- Pick out monoclonal bacterial
- Colony identification
- Linearized plasmid of pPIC9K
- Clean and purify the restriction enzyme digestion products
- Preparing competent yeast cells
- pPIC9K transformation of GS115
- Yeast Inducible expression
- Oxford Cup antimicrobial test
- Tris-Tricine SDS PAGE electrophoresis
- Antimicrobial evaluation of Plectasin NZ2114 peptide by growth curve
- Cell-titer to evaluate the activity of Plectasin NZ2114
- SEM (Scanning Electron Microscope) assay