Parts

Table of Contents

1. Overview

Throughout the engineering design cycles of our project, team HAOLVE-Nanjing designed and characterized 4 new basic parts and 6 new composite parts. Our goal was to enhance the rare earth elements (REEs) adsorption capacity of Pseudomonas kunmingensis (P. kunmingensis) HL22-2T by genetically reprogramming its inorganic polyphosphate (polyP) metabolic network. This synthetic biology approach aims to advance more efficient and sustainable REE recovery processes.

2. New Basic Parts

We registered and uploaded basic parts BBa_25WRSMIP, BBa_25CVSUA1, BBa_25TC4IDA.

We also uploaded basic parts BBa_259STERK as a backbone and proved it worked as we expected.

3. New Composite Parts

We have designed eight composite parts (BBa_2502RTST, BBa_253PFMUM, BBa_25J1CVVU, BBa_25WN4TIV, BBa_259D2DLA, BBa_2566HVYF) in order to overexpress the corresponding genes in cells.

Part Number Part Name Part type Abstract
BBa_25WRSMIP P.KPPK1 Coding Catalyzing the reversible transfer of the terminal phosphate of ATP to form a long-chain polyphosphate (polyP) from Citrobacter Freundii.
BBa_25CVSUA1 P.KPPK2-Ⅰ Coding Using inorganic polyphosphate (polyP) as a donor to convert GDP into GTP and ADP into ATP from Pseudomonas aeruginosa.
BBa_25TC4IDA P.KPPX Coding Catalyzing the degradation of inorganic polyphosphate (polyP), progressively releasing orthophosphate from the terminal ends of polyP chains from E. coli.
BBa_259STERK Modified T7 RBS-P.KPPK1 Device Catalyzes the reversible transfer of the terminal phosphate of ATP to form a long-chain inorganic polyphosphate (polyP). This part is activated by a modified T7 RBS (BBa_K4604004).
BBa_2502RTST Modified T7 RBS- P.KPPK2-I Device Catalyzing the synthesis of GTP or ATP from polyP. This part is activated by a modified T7 RBS (BBa_K4604004).
BBa_253PFMUM Modified T7 RBS-P.KPPX Device Catalyzing the degradation of polyP. This part is activated by a modified T7 RBS (BBa_K4604004).
BBa_25J1CVVU pCM28 Device An arabinose-inducible vector plasmid backbone with ampicillin resistance. With this device, we can insert the coding sequence of a novel protein.
BBa_25WN4TIV pCM28-Modified T7 RBS-P.KPPK1 Plasmid We loaded the genes encoding PPK1 into plasmid vectors. In this way we were able to over-express the BBa_25WRSMIP in the target cells.
BBa_259D2DLA pCM28-Modified T7 RBS-P.KPPK2-Ⅰ Plasmid We loaded the genes encoding PPK2-I into plasmid vectors. In this way we were able to over-express the BBa_25CVSUA1 in the target cells.
BBa_2566HVYF pCM28-Modified T7 RBS-P.KPPX Plasmid We loaded the genes encoding PPX into plasmid vectors. In this way we were able to over-express the BBa_25TC4IDA in the target cells.