Overview
Our team has always attached great importance to laboratory safety and strictly abides by the Safety Specifications for Laboratories in Institutions of Higher Education. Faced with many samples and reagents, we implement safety regulations, focus on safety management, and strengthen safety education to ensure the health and safety of every student involved in laboratory work. With joint efforts, the team has successfully completed all experiments safely.
General Safety
Laboratory Environment
Project Design Safety
Microbial Safety
- When using Bacillus velezensis and Escherichia coli, relevant wastes such as cultures and bacterial solutions must be autoclaved at 121°C for more than 30 minutes. After confirming sterility, they should be treated as medical waste. Contaminated utensils and consumables should be cleaned or discarded after sterilization.
Chemical Reagent Safety
- Chemical reagent safety: When handling kanamycin, avoid cross-contamination caused by residues; clean thoroughly after operation. Waste liquid containing kanamycin should be collected separately, labeled "Antibiotic Waste Liquid", and handled as hazardous waste by professional institutions to prevent the spread of drug-resistant genes.
- Waste liquid, pipette tips, gloves, etc., contaminated with nucleic acid dyes should be collected separately, labeled "Toxic Waste Liquid", and handed over to professional institutions for disposal.
Laboratory Safety
Requirements for Personal Protective Equipment (PPE)
- Wear appropriate lab coats; avoid shorts, short skirts, open-toed shoes, or clothing exposing skin.
- Tie up long hair; remove rings/bracelets to prevent entanglement or contact with reagents.
- Use protective equipment as needed: chemical-resistant gloves for chemicals; masks, face shields when handling infectious biological materials.
Code of Conduct
- No eating or drinking in the lab; avoid touching mouth, nose, or eyes with hands.
- Items unrelated to experiments are not allowed on the bench; keep it clean and tidy.
- Do not leave during experiments; if necessary, turn off fire, power, and reaction devices before leaving.
- After experiments, wash hands, clean benches, and turn off water, electricity, and gas.
Requirements for Chemical Storage
- Store chemicals by category.
- Flammable/explosive substances in explosion-proof cabinets; highly toxic chemicals are double-locked and usage is recorded.
- Label containers with name, concentration, purchase date, hazards.
- Volatile reagents in fume hoods; low-temperature reagents in special containers.
- Waste chemicals into labeled waste barrels; solids into special bins; sharps into puncture-proof boxes; handled regularly by professionals.
Electrical Safety
- Do not touch power switches or sockets with wet hands.
- Arrange wires neatly near benches to avoid tripping or soaking in water.
Safety Training and Management
Before all experiments, students completed systematic safety preparation: two semesters of chemistry experiment courses and special training on the safe use of ultra-clean benches, biosafety cabinets, fume hoods, and PCR instruments. Regulations for biological and chemical operations were explained in detail. During experiments, senior team members or teachers were present for assistance and supervision.
