Parts | Name | Type | Function | Results |
FLG | Coding gene | The filaggrin (FLG) is a major epidermal protein that ensures the correct formations and function of the skin barrier. FLG can be broken down into natural moisturizing factors(NMF), including urocanic acid (UCA) and pyrrolidone carboxylic acid (PCA), which keeps the skin hydrated. [1] | Confirmed in DH5α, B(DE3)pLysS strains and fibroblast cells. | |
FLGxsc | Coding gene | The filaggrin (FLG) is a major epidermal protein that ensures the correct formations and function of the skin barrier. FLG can be broken down into natural moisturizing factors(NMF), including urocanic acid (UCA) and pyrrolidone carboxylic acid (PCA), which keeps the skin hydrated. [1] | Confirmed in DH5α cells. | |
RFP | Coding gene | Fluorescent proteins (FPs) allow the specific and targeted labeling of proteins, organelles, cells, tissues, and whole organisms. This versatility makes FPs superior over synthetic organic dyes for imaging applications in vivo. [2] | N/A | |
PLrigid | Protein domain | PLrigid is a 20 a.a. peptide that is based on a previously characterized alpha-helix motif (EAAAR) [3] | N/A | |
pET-28a(+) | Plasmid backbone | pET28a(+) is a nonviral expression plasmid used in bacteria strains for protein production purposes. It has kanamycin resistance gene as a selection marker. Plasmid sequence consists of T7 promoter and terminator sequences as well as lac operon sequence and lacI promoter for protein expression induction. It has both N- and C- terminal HisTag sequences and N- terminal thrombin cleavage site. | Confirmed in DH5α and B(DE3)pLysS strains |