Theory course: Background introduction to the iGEM challenge

1. Theory course: Basic research methods of modern molecular biology.
2. Topic discussion: Briefly introduce igem background and scoring criteria, give topic

selection and introduction

3. Theory course: Social Insight and Market Exploration: The art of questionnaire research and interview

1. Project discussion: Give a project selection speech and vote for the final project.
2. Theory course: The Art of popular Science Communication: Creating a concise and logical scientific dialogue
3. Theory course: From 0 to 1, the Road to Product Commercialization: Business planning and product Landing strategies.

1. Project background Review
2. Team discussion: team name, Logo, team uniform, etc
3. Laboratory safety training
4. Experiment conducted：

1. LB medium preparation
2. Glycerin inoculator
3. Plasmid extraction
4. The target gene PETase/MHETase was amplified by PCR
5. DNA identification by agarose gel electrophoresis
6. DNA glue recovery
7. Homologous recombinant DNA and vector
8. Convert E.coli DH5α/BL21
9. Plate coating screening
10. The transformant was identified by PCR
11. Select monoclonal culture seed solution
12. Extended culture

1. Finalization of education scheme（kindergarten, primary school, middle school, high school, public）
2. Create a team account on social media platforms
3. Make and publish a questionnaire about plastic degradation
4. Plan on-campus promotion (post posters, design small games)

1. Design business plan
2. Expert Interviews: Dr. Chen Guangquan, Dr. Li Shiyuan
3. Write a verbatim transcript of the interview
4. Discuss the progress of the current project and the areas that need improvement

1. Write the script for the promotion video
2. Shoot the imitative promotion video of the competition team
3. Sort out the data obtained from the questionnaire survey
4. High school education: Lecture on Plastic-Degrading Enzymes
5. Elect the team leader, the leader of the wet team and the leader of the dry team

Learn the basic knowledge that the wet team used in the subsequent experiments：

1. NCBI gene mining
2. AlphaFold predicts protein structures
3. Molecular docking predicts the binding effect
4. Homologous recombination and plasmid construction of eight variants

Wet team group report:

1. Make a preliminary division of labor for the content writing of the Wiki website
2. Kindergarten education：puppet show -- Enzyme Hero's Magic Scissors

1. Primary school education: Lectures + Handicraft Activities
2. Finalize the design style of the wiki
3. The shooting of the promotion video

Complete the editing of the promotion video and upload it to the website

Dry team：

1. Create pull-up banners and prepare the planning proposal for the 8.27 community education
2. The IHP survey
3. Summarizes Dr. Li Shiyuan's presentation content and gains, integrates them and completes the IHP
4. Community education prepares and questionnaires
5. Prepare the interview outline for Professor Huo
6. Wiki writing for primary school education
7. Community education：lectures, promotions, games
8. Interview Outline for Professor Shan
9. Introduction to Education Community wiki Activities
10. Professor Shan's IHP reflection
11. Interview with Professor Huo

Wet team：

1. Review the content of the research topic and the intensive training,
2. The expansion culture of the strain, the determination of OD600, the creation of growth curves
3. The induction of protein expression by IPTG
4. Expand the culture of the strain, OD600, induce protein expression.
5. Ultrasonic disruption of cell walls
6. Learn the principle of nickel column purification and protein purification
7. Divide the tasks of the flowchart
8. SDS-PAGE protein isolation
9. Learn the principle of HPLC
10. Prepare samples of PET degraded by PETase, and take samples for HPLC testing after 48/72 hours
11. Protein purification
12. The remaining proteins from yesterday were identified, stained and decolorized, and Prepare HPLC samples
13. The synergistic effect of PETase and MHETase was identified using PET film
14. Draw flowcharts（BioRender）

Wiki：

1. Write content for the wiki website
2. Make the first version of the ppt for the project defense
3. Revise the project defense ppt
4. Conduct the first mock project defense