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Parts

The core advantage of synthetic biology lies in its capacity to efficiently construct complex biological systems via the "building block assembly" strategy—different biological components within this framework serve precisely as the core "building blocks" for implementing this strategy. These components exhibit standardization and well-defined functions, and can be flexibly combined according to research and design requirements, which can then be assembled into target-specific functional modules. Such functional modules can precisely align with design objectives, thereby transforming abstract research concepts into actionable biological functions and providing critical support for the construction and application of complex biological systems.

Basic Parts

We have primarily adopted several basic components, with lactase (β-galactosidase) and lactose pump (lactose permease) serving as the main components, along with the pGAP promoter, LuxS (BBa_25D94UKL), and MucBP (BBa_25MHNS1O). These components are used to optimize our Lacbutler system, enabling it to achieve better colonization in the intestine and enhanced biological safety.

Part name Part type Short Description
BBa_25Y6UBUT Promotor pGAP : Two-strain compatible compositional promoter
BBa_K3416001 Promotor pEP01rec : Ai-2-induced promoter
BBa_25G9QZPK Promotor pbreAB : BreR - inhibited promotor
BBa_K4062008 Protein Domain LacY : Lactose permease
BBa_25D94UKL Protein Domain LuxS : S - ribosyl homocysteine lyase
BBa_K5127002 Protein Domain BreR : An allosteric TetR-family transcription
BBa_25RS33SI Protein Domain BgaB : β-galactosidase
BBa_25MHNS1O Protein Domain MucBP : adhesion protein
BBa_25RUY7II Protein Domain CcdB : toxic protein
BBa_254LO6HW Plasmid pMB1-A: Shuttle plasmids of Bifidobacterium and E.coli

Composite Parts

Furthermore, the use and construction of composite components can effectively realize our vision for this project of "density self-regulation and function self-implementation".

Part name Part type Short Description
BBa_25F5CNJA Composite Lactose Uptake System
BBa_25DNDNFN Composite High-Efficiency Lactose Catabolism System
BBa_25MXB4QV Composite Intestinal DCA (Deoxycholic Acid) Specific Sensing System
BBa_25NP6D0N Composite Intestinal Adhesion System
BBa_258EH4JM Composite Endogenous AI-2 Provision System
BBa_25GLVD1S Composite AI-2 - Mediated Suicide System

We anticipate that these carefully designed components will not only play a crucial role in the advancement of this project but also contribute significantly to the broader iGEM community and the sustained development of synthetic biology as an interdisciplinary field.

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