In direct fulfillment of our project goal, we have successfully laid a robust foundation for constructing a thermotolerant microbial platform for specialized polyamine production.
The key conclusions are:
1. Validated the Chassis Strategy: We identified and characterized native thermotolerant Bacillus strains and confirmed that their heat stress response intrinsically involves the upregulation of polyamine synthesis pathways, making them ideal chassis candidates.
2. Decoded Resistance and Biosynthetic Mechanisms: Through integrated multi-omics and morphological analyses, we uncovered the core mechanisms of heat stress resistance—encompassing metabolic, proteostatic, and structural adaptations—and defined the specific role of polyamine metabolism within this network.
3. Identified Key Synthetic Biology Elements: We mined and phylogenetically characterized critical biosynthetic genes (e.g., for spermidine synthase) from high-temperature microbiomes, providing a essential genetic toolkit for future engineering.