Overview

In this study, we contributed 10 new parts to iGEM, including 5 basic parts and 5 composite parts. Using these 10 new parts, along with 4 previously existing basic parts, we successfully expressed the AsCpf1 (Cas12a) protein and prepared standard simulated samples for detecting the c.520G>A and c.6745C>T mutations in the COL7A1 gene, which are associated with Dystrophic Epidermolysis Bullosa (DEB). Building on these components, we developed fluorescence detection and test strip detection platforms for the c.520G>A and c.6745C>T mutations, using RPA and engineered Cas12a for expression and purification. These platforms represent a novel, portable, low-cost, and low-equipment-dependent solution, offering a significant contribution to society by improving disease detection rates, enabling early diagnosis, and supporting clinical decision-making, ultimately benefiting human health.

The relevant parts are listed in Table 1. Among them, the first four (BBa_K3521000, BBa_K3521002, BBa_K3521004, BBa_K3584001) are pre-existing parts, while the others are new parts contributed by this study.

Table 1. The relevant parts.