Overview

Safety is the top priority in our laboratory. We have emphasized safety established the following safety guidelines, whose importance is to prevent the release of genetically modified microorganisms and hazardous chemicals, ensure the safety of personnel and the environment, and enable responsible and sustainable research.

1. Personal protection & lab management

General ab ygiene: No eating, drinking, or smoking in the lab. Always wash hands thoroughly with soap before leaving the lab. Return equipment after use, to  workspaces cleanAlways be vigilant with fire hazards and electric shock dangers

Personal Protective Equipment PPE: Lab coats loves must be always worn in the laboratory and  before touching common items (e.g., door handles, phones). Safety glasses must be worn when there is a risk of splashes.

Emergency PreparednessKnow the locations and operating procedures for the emergency eye washing station and safety shower. 

Chemical or biological spill mmediately contain the area apply an appropriate disinfectant. For skin contact, flush immediately with water.

Aemergencies reported to the lab supervisor or safety officer immediately.

2.Biosafety

Our laboratory is a Biosafety Level 1 facilityAll s involving E. coli H5α or BL21(DE3) must be performed within a Biological Safety Cabinet (BSC).

NThe strain used in our experiments  E. coli DH5α BL21(DE3). Tcommon laboratory strain that ha a low survival rate in human tissues and virtually no potential to cause pathogenic infection when handled carefully.

Change the pipette tips for different reagents/samples to avoid cross-contamination

Waste Disposal: All consumables (pipette tips, tubes) and all biological waste must be decontaminated before disposal. Never pour cultures down into the drain.

3. Chemical Safety

All reagents should be labeled with their name, concentrations, and dates to prevent misuse. Hazardous reagents  not be disposed of by individuals privately. The hazardous   our experiment  listed as follows.

1.  irritant and 

Acrylamide: a highly toxic monomer and neurotoxic agent.

Bis-acrylamide: a cross-linking agent with associated neurotoxic.

APAmmonium persulfateinitiator of polymerization high reactivityombustible and irritant.

TEMEDN,N,N',N'-Tetramethylethylenediaminea catalyst for free radical polymerizationlammablecorrosive and irritant.

an anionic detergent widely used for its strong denaturing and solubilizing effects on proteinsrritant  skin eye and respiratory tract.

bromophenol blue, and  a reducing agent and potent denaturant with a highly offensive odorhighly toxic, volatile, and characterized by its repulsive smell.

2. EB

Ethidium bromide a compound that intercalate into double-stranded DNA  RNAwidely used as a fluorescent  for nucleic acid detection in gelshighly mutagenic

a purified polysaccharide derived from algae agar low chemical reactivity.

Acetic acid pungentEthylenediaminetetraacetic acid low toxici

3. Kanamycin 

a broad-spectrum antibiotic. Ivoid cotoxicity antibiotic resistance spread ab ersonnel allergies

a natural polymer extracted from red algae non-toxic.

4. ent coffee grounds

A

1.  electrophoresis buffer EB

2.  electrophoresis buffer Glycine

Glycine: low-toxicity amino acid and buffer. 

3. Liquid Waste 

a organic solvent highly flammable and volatile.

4. Liquid Waste 

Sodium hydroxide, a highly corrosive strong base.

5. 

a synthetic inducer for lac-based expression bacterialow toxicity

Caffeinea methylxanthine alkaloidlow toxicity

ZnSO₄ FeSO₄MgSO₄NaNO₃NH₄Clsoluble saltlow toxicity

Ureaa diamide of carbonic acid and a terminal product of nitrogen metabolismlow toxicityMpour down into the draint  increase the nitrogen load eutrophication of the water body.

Basically non-toxicautoclave

All chemical waste must be sorted and over to  qualified chemical waste company for final disposal.

4.Operational Safety

Biological Safety CabinetKeep away from BSC when UV disinfection

Autoclave: Must be operated by trained personnel. Do not autoclave flammable or corrosive materials. Do not leave the autoclave unattended and ensure the pressure has dropped to  before opening the door.

Centrifuge: Always balance  tubes before centrifugation.

Electrophoresis Equipment: Wear gloves when handling gels containing nucleic acid dyes. To avoid overloading the circuit, never electrical equipment with wet gloves. Dispose of gels and buffer waste according to chemical safety .

Water Bath Prevents h burnsLhot s/bottles cool  before handling samples

5. Product safety

All fertilizer products have undergone sterilization to ensure that there is no risk of microbial leakage into the environment.