Overall Scheme
Our notebook documents the entire journey of our team throughout the 2025 iGEM season. It records our daily activities, experiments, discussions, and decisions in chronological order. This detailed record reflects both our scientific process and our teamwork.
Week1
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Week2
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Week3
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Week4
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Organizer/ Participator
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January
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Winter Holiday - Post recruitment information |
Ke Liu |
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February
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Team Formation - In-person interview |
Ke Liu, Ling Li,Chenguang Shen |
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March
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• Group cooperative learning • Research on the iGEM Website |
• Select the track and theme; Brainstorming;Designed expertments【Wetlab & Drylab】 • Integrate existing resources; Built the HP framework【HP】 • Select the design style; Design the team flag and emblem【Art Group】 • Learn how to build a web framework by referring to the best wiki teams【Wiki】 |
• Brainstorming; Determine the specific parts and Protocols【Wetlab & Drylab】 • Refine the work details in the directions of ihp, education, and inclusivity【HP】 • Modify the PPT template【Art Group】 • Learn front-end technology【Wiki】 |
All team members |
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April
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• Obtain chassis bacterial; Purchase experimental consumables【Wetlab】 • Epidemiological investigation model【Drylab】 • Design interview outlines for clinicians, patients (and their families); Design questionnaires【HP】 • Picture book and short video production; Design iP and wiki animation【Art Group】 • Learn Mini Program game development【Wiki】 |
• Guangzhou Regional Exchange Conference of iGEM Greater Bay Area Synthetic Biology Industry-Academia-Research Forum Successfully Held at Southern Medical University • Plasmid construction;Prepare for on-site report;Consulting expert【Wetlab】 • Model fitting【Drylab】 • Interviews Clinical doctor/Patient (and family member)/Enterprise; Promotion of Rural Science Popularization Work in Zhaoqing【HP】 • wiki Page design;Prepare the roller banner and peripherals【Art Group】 • Mini Program game development【Wiki】 |
All team members |
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May
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• The initially selected protein structure of H1N1; Protein-protein docking prediction【Wetlab & Drylab】 • Synthetic Biology Open Day Event; Science experiment kit; Popular Science Video Proposal; Colorblind and blind-friendly design; Planning in the direction of women's rights protection【HP】 • Design wiki page【Art Group】 • AR picture book;Complete the web page【Wiki】 |
• Preliminary Project Safety Form; Village Selection; Growth curve plotting【Wetlab】 • AlphaFold constructs the structure of the medi8852 antibody; extracts docking energy【Drylab】 • Second Round Biosynthesis Open Day; Science popularization video making; Synchronously update activity records on various media platforms【HP】 • Excellent video analysis for Project Promotion Video; wiki page design【Art Group】 • Wiki page development【Wiki】 |
• The 9th iGEM South China Exchange Conference • Primer Ordering; Project description; Consumables Confirmation & Order Placement【Wetlab】 • medi8852 scfv sequence ligand construction of 3D model; Protein docking of medi8852 and h1n1【Drylab】 • Interviews; Advance Women's Rights Protection (Questionnaire Collection and Interviews)【HP】 • Design team uniforms; Collection and production of creatives for the project promotion video【Art Group】 |
All team members |
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June
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• Electroporation transformation【Wetlab】 • Try rigid docking, zdock; Find the literature docking checkpoint and further perform flexible docking【Drylab】 • Improve the HP framework; Questionnaire Collection and Data Analysis; Phase III Open Day Collaboration; Proposals to improve the needs of international students【HP】 • Production of iGBA Conference Pass (Seed Paper); Promotion video script【Art Group】 • Upload wiki; fix bugs【Wiki】 |
• Contribution Planning; CCIC presentation PPT; shoot a promotion video【Wetlab】 • Optimize zdock modeling - mdockpp Calculate docking dynamics; Fitted growth curve OD value【Drylab】 • Science popularization tweet; Hundred-Thousand-Million Project; Interview with an obstetrician-gynecologist【HP】 • Poster production; Design a pregnancy epidemic prevention kit; Pregnancy Handbook Production 【Art Group】 • Upload wiki; fix bugs【Wiki】 |
All team members |
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July
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• Summer iGBA Forum: Where Innovation Meets Responsibility • Write the parts content; Design the safety content; Electroporation & PCR【Wetlab】 • IHP feedback mechanism; Organize the text and pictures of the activities that have been carried out【HP】 • Promotion video editing; animation production; AR picture book layout; homepage production; Create visual charts and their animations【Art Group】 • Upload wiki; fix bugs【Wiki】 |
• Safety & Security Page; Part documentation completed; Design Engineering【Wetlab】 • seiar model【Drylab】 • Organize the list of stakeholders; attribution; team members information collection; Questionnaires for medical students, patients; education/inclusivity wiki page; Organize the results of legislative direction【HP】 • Edit promotion video; Create wiki creatives【Art Group】 • Upload wiki; fix bugs【Wiki】 |
All team members |
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August
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• The 12th China Conference on iGEM (CCiC) • Parts Upload; Improve the Contribution page; Write the design interface【Wetlab】 • Calculate the transmission rate of antibody use based on antibody IC50; Calculate the changes in the solubility curve of scFv at different pH values; Rationality of applying CCD modeling to predict different pH/temperature reaction interfaces in LGG engineered bacteria【Drylab】 • Organize creatives and design the ihp-wiki page; Popular science series work; Community street stall promotion; Women-themed interview 【HP】 • Three-school picture book collaboration; wiki design【Art Group】 • Upload wiki; fix bugs【Wiki】 |
• Check-in form; Final safety form; Electroporation & PCR【Wetlab】 • Randomization added to SEIAR model parameter modification【Drylab】 • Street vending in Tianhe District; Organize collaboration; National University of Defense Technology White Paper; Women's rights program advancement 【HP】 • Presentation video pre-production/brainstorming; Team members page design; Popular Science Tweet Series, Issue 6【Art Group】 • Layout of the home page; Code of the member page; Sidebar; Text; Input for other pages【Wiki】 |
All team members |
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September
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• Changes in bacteria under different virus titers with viral load as a variable; PCR; electroporation of two plasmids; Virus incubation for fluorescence measurement; Temperature-induced detection of EGFP expression; Expression of light-GFP【Wetlab】 • RNA thermometer design; write wiki page,Project Software【Drylab】 • Guangzhou Library Lecture; write wiki page;Multilingual (translated picture book)【HP】 • Design wiki page【Art Group】 • Upload wiki; fix bugs【Wiki】 |
• Supplementary Experiments • Project Software • Wiki Design and Documentation • Wiki Upload • Judging Form |
All team members |
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October
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• Wiki Design and Documentation • Wiki Upload • Judging Form • Presentation Video |
• Presentation Video upload |
• Defense Preparation |
• Participation in the 2025 Grand Jamboree |
Weekly Task Arrangement
Between March and October, our team leader, Ke Liu, coordinated weekly meetings with each subgroup. During these sessions, the specific tasks for the following week were discussed and documented. The shared work document ensured transparency and collaboration, as all team members could access, edit, and upload their progress. This systematic approach to project management helped the team maintain clear communication, effective coordination, and steady progress throughout the season
Weekly task records (PDF uploads):
Wet Lab Detailed Timetable
In addition to the overall scheme, a more detailed timetable for the wet lab stage of the project can be found below.

Image Gallery of the Experiments





