For each experiment conducted throughout the course of our research journey, we have included a PDF of the protocol that we used. Click on different experiments to see how they were conducted!
BACTERIA LABS
Oligo Annealing & Ligation
HiFi Assembly
Transformation Into 10-Beta Cells
Transformation Into Rosetta, BL21, W3110 Cells
Plasmid Isolation (Miniprep)
DNA Ligation For Cohesive Ends
Post Enzymatic Reaction Cleanup (PCR Cleanup)
PROTEIN LAB
Cas13a Expression & Purification
Cas13a-DTA-gp120 Assay
MAMMALIAN CELL LAB
Passaging
Transfection: Calcium Chloride
Transfection: Lipofectamine
Establishing a Stable Cell Line
Pelleting Cells
Post-Transfection Identification of Fluorescence Under Microscope
