Our project aims to develop a rapid, ultra-sensitive, highly specific and user-friendly point-of-care testing (POCT) platform to revolutionize the field of food safety monitoring. The core of this platform is to construct a four-in-one nanoflower system, which integrates phage-derived binding protein (PBP, for specific recognition), horseradish peroxidase (HRP, for catalyzing signal generation), gold-platinum nanozymes (AuPt, for synergistic signal amplification), and calcium phosphate matrix (CaHPO₄, as a high surface area scaffold). This design enables the completion of the test within 30 minutes, with a detection limit as low as 10 CFU/mL, and can accurately distinguish target pathogens (such as E. coli), even in complex matrices like milk and tea, and can work reliably. The results can be quantified either by visual reading or by analyzing RGB values on a smartphone, significantly enhancing the application potential in resource-limited environments. Among them, PBP is crucial for the specific detection of bacteria. In this project, we provided a total of 4 new parts, and added new experimental data of two existing parts. The relevant parts are listed in Table 1.
| Part number | Part name | Diagram | Part type | Part type |
|---|---|---|---|---|
| BBa_25PDUACA | Tail Fiber Protein |  |
Basic part | New part |
| BBa_25ZM5M81 | pET28a-TFP |  |
Composite part | New part |
| BBa_256Y31R2 | BSCBD |  |
Basic part | New part |
| BBa_25JHJB24 | pET28a-BSCBD |  |
Composite part | New part |
| BBa_K5532001 | gp17 |  |
Basic part | Existing part |
| BBa_K5532004 | pET28a-gp17 |  |
Composite part | Existing part |
