Overview

Prior to commencing the experiment, all team members participated in a safety training session to establish a foundation in laboratory safety protocols. The training covered critical topics, including lab access regulations and conduct, emphasizing guidelines for appropriate attire and the strict ban on eating or drinking in the lab. We gained insights into the distinction between biosafety levels and corresponding precautions. Instruction was provided on the proper operation of biosafety equipment (e.g., biosafety cabinets) and microbial techniques to minimize contamination. Additionally, we received guidance on effective disinfection and sterilization procedures to maintain a hygienic workspace. The session also addressed transport protocols for biological materials, as well as physical and personnel biosecurity measures. It is crucial to ensure all biological materials are safe, non-toxic, and only handled after strict sterilization. Finally, we were briefed on chemical management, fire safety, and electrical safety to ensure preparedness for safe and responsible laboratory work.

Biosafety Committee

The biosafety committee comprises one lead instructor and one research assistant. Their primary duties include ensuring team members’ safety, maintaining workspace hygiene, and providing safety training to foster a secure working environment. They also oversee safety during experiments and ensure proper equipment usage.

Safety Training

All team members must strictly adhere to lab safety rules, instructions, and procedures to ensure safe experimentation:

       1.    Practice aseptic techniques to avoid contamination and use appropriate sterilization methods for equipment and waste disposal.

       2.    Familiarize themselves with evacuation routes and emergency protocols.

       3.    Prohibit eating or drinking in the lab.

       4.    At least two individuals must be present for procedures involving hazardous substances; personnel must remain at their stations and disinfect work surfaces promptly after task completion.

       5.    Shut off utilities and restore equipment after experiments.

     6.    Comply with national safety regulations by wearing appropriate lab coats at all times; specialized gloves are required for specific chemical or biological procedures.

       7.    Report all spills, injuries, or equipment malfunctions immediately.

       8.    Ensure emergency exits and evacuation routes remain unobstructed at all times.

       9.    Tie back long hair and secure loose clothing or accessories.

       10.  Clearly label and segregate synthetic organisms to prevent accidental use or release.

       11.  Wash hands thoroughly before and after experiments.

       12.  Maintain strict lab hygiene by prohibiting storage of non-essential items, clutter, or waste.

      13.  Know the location and proper use of safety equipment (e.g., fire extinguishers, eyewash stations, emergency showers, first-aid kits, spill kits).

This protocol safeguards personnel, prevents accidents, and maintains operational integrity.

Work Area

General microbiology laboratory

Microorganisms

Escherichia coli DH5α, Escherichia coli BL21

Risk Assessment

Biological Safety:
Risk	Risk Management
Escherichia coli DH5α	Wear gloves, disinfect surfaces before/after use, wash hands
Escherichia coli BL21	frequently, and work in a biosafety cabinet.
Chemical Safety:
Risk	Risk Management
100% ethanol	Wear gloves, a mask, and eye protection.
Sodium dodecyl sulfate (SDS)	Wear gloves, a mask, and eye protection.
Hydrochloric acid	Wear gloves, a mask, and eye protection.
Acrylamide	Wear gloves, a mask, and eye protection.
Tetramethyl ethylenediamine	Wear gloves, a mask, and eye protection.
Coomassie Brilliant Blue	Wear gloves, a mask, and eye protection.
Physical Safety:
Risk	Risk Management
Hot metal bath	Wear gloves.
Centrifuge	Ensure the lid is closed and masses are balanced befor operation.
Sharp objects	Handle with care, keeping away from the body and others to
	prevent injury.
UV light (carcinogen)	Close UV transilluminator lids/shields before switching
	on; ensure biosafety cabinet doors and interlocks are functional
	pduring UV sterilization.

Tools Management:

Before formal experimentation, all personnel must attend safety training to understand equipment-related hazards. Identify safety instructors, know emergency contact information, and contact the lab safety supervisor immediately in case of urgent issues.

Reflection

Ensuring every researcher’s safety is a prerequisite for conducting experiments. Our laboratory fully complies with national and legal requirements, and we remain committed to adhering to these protocols.