With the increasing global risk of cardiovascular diseases, salidroside has been found to be a reliable component that can relieve the pressure on the heart. Our team plans to utilize synthetic biology technology and use Escherichia coli as the chassis organism to synthesize salidroside. This method can significantly increase the yield of salidroside, reduce costs, and also aligns with the concept of sustainability.
Our team plans to establish a health supplement company and launch a series of dietary supplements based on salidroside. These products are designed to assist the public in maintaining cardiovascular health, alleviating physical fatigue, and supporting overall wellness,serving as a daily health maintenance solution.

Research data shows that in 2016, the number of people working more than 55 hours a week worldwide had reached 488 million (accounting for approximately 8.9% of the global population). From 2000 to 2016, the proportion of this group increased by 9%. The proportion of the global working population working overtime generally fluctuates between 8% and 10%. Over the past 16 years, it has increased by 9%, with an average annual growth rate of 0.56%(Figure 2). Conservatively estimated, by 2030, the number of people working overtime will increase by 7.84%, possibly reaching around 880 million. The number of the working population in 2024 is even larger (5.68 billion), so the number of people working overtime may be higher than estimated.

All long-term high-pressure work directly leads to problems such as insufficient sleep and disrupted schedules. What's even more terrifying is that it increases the risk of death from cardiovascular and cerebrovascular diseases by 52%, becoming an "invisible killer" threatening human health. With the solidification of the working mode, this problem is worsening year by year. According to the report of the WHO, if not intervened in time, it will cause irreversible damage to public health (Figure 3). At present, many products aimed at supporting cardiovascular health, including pharmaceuticals and supplements, face challenges such as high prices, potential side effects, and concerns regarding long-term safety and efficacy. Some drugs, when taken for a long time, may also raise blood sugar and cholesterol levels and lead to related diseases.

For instance, a survey of the current health supplement market reveals that high-quality Rhodiola rosea supplements command a significant price premium.As shown in Figure 4, the price of these Rhodiola-based supplements generally ranges from around $13 to $27 per bottle, with some premium options being even more costly. The cheapest one is Rhodiola rosea capsules, which cost only 15 to 20 yuan per box and are quite cost-effective. However, they are positioned differently, primarily targeting specific physiological states rather than general wellness supplementation.
In the process of addressing cardiovascular health challenges, salidroside has gradually come into the research field. This active ingredient extracted from *Rhodiola rosea* has been confirmed by multiple studies (including those from Dalian University, the China Tibetology Research Center, and China Pharmaceutical University, etc.) to support overall cardiovascular wellness. It is known to aid in healthy circulation, thereby contributing to one's general sense of vitality andwell-being.Whether it is for the cardiovascular burden caused by high-pressure work or the common cardiovascular degenerative problems among middle-aged and elderly people, salidroside has demonstrated unique maintenance value and has become a potential breakthrough for preventing and improving related health issues. In addition, salidroside also has multiple functions such as antioxidation, anti-inflammation, regulation of vascular function and improvement of neurocognitive function, providing a broader prospect for its application in the health field (Figure 5).
However, the traditional plant extraction method, which currently accounts for 72% of the salidroside market, faces numerous challenges, including high costs, unsustainable resources, insufficient stability of product efficacy, and limited preventive options. Rhodiola rosea, as a raw material, has an extremely limited supply due to its growth cycle of 4 to 5 years and its listing as a "key protected variety of Chinese medicinal materials". In 2024, the raw material price of Rhodiola rosea has soared to 82 RMB per kilogram, while the content of salidroside in each Rhodiola rosea plant is only 0.8-1%, resulting in an extraction cost as high as approximately 30,000 RMB per kilogram. These restrictions have severely restricted the commercial application of salidroside.
To break through these bottlenecks, we plan to adopt synthetic biology technology and use Escherichia coli as the chassis organism to achieve efficient synthesis of salidroside. Compared with the traditional plant extraction method, this method can achieve a salidroside yield of 17.36 milligrams per hour, significantly enhancing production efficiency and reducing the cost to approximately 40 RMB per liter. In addition, synthetic biology technology does not rely on scarce Rhodiola rosea resources, which is in line with the concept of sustainable development.

1. Some traditional health-regimen options are costly and controversial: long-term use imposes heavy financial burdens, and some consumers have concerns about their suitability for daily prevention.

2. Natural salidroside faces an ecological bottleneck: wild Rhodiola is a China-class-II-protected species; over-harvesting endangers fragile alpine ecosystems and drives raw-material prices ever higher, blocking large-scale supply.

3. Prevention is largely ignored: current solutions focus on treatment, leaving high-risk groups―overworked adults and the elderly―without safe, affordable, and easy-to-use daily dietary supplements for cardiovascular wellness.

First, we conducted a survey among people of different age groups, including purchasing motives, preferred formats of nutritional products and purchasing channels. To understand the diverse demands of people of different ages for nutritional and health products. The FDA and AAP and other institutions all emphasize that it is not recommended for minors to use supplements containing herbal ingredients. Therefore, the subjects of our investigation were all adults over the age of 18.
Due to differences in living conditions and health needs, people of different age groups show distinct characteristics in the consumption of nutritional and health products(Figure 6). The specific profiles are as follows:

• Aged 18-24: Young adults prioritize fast, portable health boosts to counter fatigue from studying and nightlife while also caring about skin health. They gravitate toward novel, ready-to-consume formats—especially beverages and oral shots. As the most internet-savvy cohort, they rely on social platforms like Douyin and Xiaohongshu for information and purchases, often making impulse buys driven by trending topics and playful marketing.

• Aged 25–34: Burdened by high work pressure and frequent overtime while maintaining regular fitness routines, this group buys the product to stay energized, combat fatigue, and support skin health. They value efficiency and convenience, preferring capsules and tablets that fit seamlessly into a hectic schedule. As the core demographic of online shoppers, they primarily purchase through e-commerce platforms, adept at comparing deals and stocking up at the click of a button.

• Aged 35–44: In mid-life, these individuals often compromise their health through late nights and business entertainment, with declining sleep quality becoming a key concern. Their primary motivation for purchase is to improve sleep and boost immunity, and they favor high-potency capsules. Cautious buyers who value trusted endorsements, they rely on reputable e-commerce platforms as well as recommendations from offline friends.

• Aged 45–54: As their bodies change with age and they travel to high-altitude destinations, they focus on cardiovascular health and altitude adaptation. Influenced by traditional wellness values and the culture of gifting, they prefer premium gift-box supplements. Purchases are made at offline pharmacies and dedicated wellness centers.

• Aged 55–64: Entering retirement, they buy to sharpen memory and slow aging. Guided by traditional wellness culture, they favor complex, multi-ingredient formulas and rely on TV shopping channels and recommendations from family or friends.

Based on the survey results, we can analyze the different demands of consumers for nutritional and health products. And based on different demands, discover the potential customer groups of our products(Figure 7).

Salidroside Market Report – China (January 2020 – July 2025)

We have analyzed the health supplements currently available on the market that can alleviate cardiovascular diseases, and summarized the advantages, disadvantages and areas for improvement of different products.

Competing product name	Advantages	Disadvantage	Opportunity point
Fish oil/American ginseng	Anti-hypoxia/anti-fatigue, stable technology	The ingredients are single, the effect is slow, and the data is insufficient	Compound formula, citing authoritative journals
Shenglian Rhodiola rosea Capsules	The target customers are clear and the effect is quick	The price is on the high side and there are risks in its use	Strengthen publicity and conduct fixed-point experiments
Coenzyme Q10 Capsules	High consumer awareness; widely used for heart health; research indicates its key role in cellular energy production.	Single-ingredient formula, limited comprehensive effects like anti-fatigue	Our product offers combined anti-fatigue and antioxidant advantages
Slices of Rhodiola rosea	Traditional medicine is safe and reliable	The operation is troublesome and the dosage is inaccurate	The product is easier to use and has a standardized dosage
Nattokinase Capsules	Strong reputation in cardiovascular maintenance	Function focuses on circulation, lacks anti-fatigue evidence	Our product meets more diverse health demands

Product Information

① Fish oil/American ginseng
Product description: Extracted from fatty fish, it is rich in n-3 series polyunsaturated fatty acids such as DHA and EPA. It is known to help maintain healthy blood lipid levels, support cardiovascular wellness, and contribute to mood balance, skin health, and joint comfort. It is available in various dosage forms such as liquid, capsules and tablets.
Manufacturers: Tongshen Bio-Health, Swisse, Blackmores, etc.
American ginseng
Product description: A plant of the genus Panax in the Araliaceae family, it was originally native to North America and later introduced to China. It has a sweet and slightly bitter taste, is cool in nature, and enters the heart, lung and kidney meridians. It contains triterpene saponins and other components, and has the functions of tonifying qi and nourishing Yin, clearing heat and promoting the production of body fluids. It can be used to treat qi deficiency and Yin deficiency, false fever and restlessness, etc. It is cultivated in places such as Huairou in Beijing and Changbai Mountain in China.
Manufacturer: Beijing Tongrentang, etc.
② Coenzyme Q10 Capsules:
Product Description: A liposoluble quinone compound present in every cell of the human body is a key component of the mitochondrial energy factory in cells. Coenzyme Q10 is abundant in high-energy-consuming organs such as the heart, liver and kidneys. It can protect cells from oxidative damage and activate cell metabolism and respiration. It helps support cellular energy production and maintain cardiovascular health, and is suitable for people with insufficient synthesis in the body due to aging.
Manufacturers: Swisse, Blackmores, etc.
③ Shenglian Rhodiola Capsules:
Product Description:Utilizing soft capsule technology, the raw materials are sourced from high-quality Rhodiola rosea at an altitude of over 3,500 meters. Certified by ISO9001 and GMP. Studies suggest Salidroside can help support healthy circulation and overall cardiovascular function, and enhance the body's tolerance to physical stress.
Manufacturers: Astrazeneca, Guangzhou Baiyunshan Tianxin Pharmaceutical, Changzhou Siyao Pharmaceutical, Farmito, etc.
④ Rhodiola rosea Slices:
Product description:Produced by Anhui Meikangyuan Biotechnology on behalf of Nanjing Tong Ren Tang, originating from Bozhou, Anhui Province, in bulk, with a net content of 60g, not a health food, and a shelf life of 530 days. The raw material is high-quality large-flowered Rhodiola rosea from Xizang, which can be used as a tea substitute or for making tea bags.
Manufacturer: The entrusting party is Nanjing Tongrentang, and the producer is Anhui Meikangyuan Biotechnology Co., LTD.
⑤ Nattokinase Capsules
Product Description: A serine protease extracted from natto through microbial fermentation technology. Nattokinase is known to support healthy circulation and help maintain normal blood flow. It is used by individuals seeking to promote their cardiovascular wellness.
Manufacturer: GeneCare,Nobel Medical Research Institute, etc.

S: Strengths:

1. Technical advantages: By applying synthetic biology technology and using Escherichia coli to synthesize salidroside, it has broken through the resource limitations of traditional extraction of salidroside from plants and is sustainable.

2. Cost advantage: Under the guidance of experts, the plan is to screen and selectively evolve key enzymes, which is expected to increase the production of salidroside and have a leading position in technology. It is expected to reduce the production cost of salidroside.

3. The product's efficacy advantages: Salidroside has multiple health benefits such as anti-hypoxia, anti-fatigue, and enhancing immunity. Compared with some single-function health products on the market, it has a wider range of applicable population and effects.

W：Weaknesses

1. Biosafety and ethics-related concerns: The application of synthetic biology technology may cause some consumers to worry about "biosafety", and at the same time, it may face ethical disputes among minority groups regarding new technologies. Such concerns may lead to a relatively low initial market acceptance.

2. The product form is monotonous: If only a single dosage form (such as capsules) is launched, it is difficult to meet the needs of different groups of people (such as children needing chewable tablets and office workers needing portable strips). It is possible to investigate user preferences, develop multi-dosage form products, and enhance market adaptability.

O：Opportunities

1. The market demand has expanded. With the improvement of people's health awareness and living standards, the demand for health products has shown a rapid growth trend. Especially for middle-aged and elderly people, those in a sub-healthy state, and people in high-altitude areas, there is a strong demand for health products with anti-hypoxia and anti-fatigue effects, which provides a market space for salidroside health product capsules.

2. The policy support is favorable. The state is paying increasing attention to the big health industry and has introduced a series of policies to encourage the development of the health supplement industry. This has created a favorable policy environment for the research and development, production and sales of salidroside health capsules.

3. Technological innovation has contributed to the continuous development and innovation of synthetic biology technology, providing new technical means and methods for the efficient synthesis of salidroside. With the continuous advancement of technology, it is expected that production costs will be further reduced, product quality will be improved, and the competitiveness of products in the market will be enhanced.

T：Threats

1. The market competition is fierce, and the competition in the health supplement market is particularly intense. There are already numerous health supplement brands and products in the market, including some well-known brands and traditional Chinese medicine health supplements with traditional advantages. Salidroside health supplement capsules are under market pressure to win in the market competition.

2. Regulatory and policy risks: The health supplement industry is subject to strict regulatory and policy supervision. Changes in regulations and policies may have a significant impact on the research and development, production, and sales of products. For instance, Escherichia coli is still not permitted for marketing.

3. The consumer trust crisis has led to a decline in consumers' trust in health supplements due to problems such as false advertising and counterfeit and shoddy products in the health supplement market.

Salidroside health supplement capsules need to establish a good brand image through quality control and market promotion, and regain consumers' trust. People's vigilance towards biosynthesis technology.(Figure 8)

Speed: Traditional plant extraction requires waiting for *Rhodiola rosea* to grow and mature, with a cycle of several years, and the extraction process is also time-consuming. Under laboratory conditions, synthetic biology technology can complete the synthesis of salidroside within several weeks. When it comes to large-scale production, by optimizing the fermentation process, batch production can be achieved rapidly, significantly shortening the cycle from production to market launch.
Sustainability: Rhodiola rosea is a second-class protected plant in China. Traditional extraction methods rely on wild resources. Excessive exploitation will disrupt the ecological balance, and the limited resources make it difficult to sustain. Our synthetic biology technology does not rely on wild Rhodiola rosea. It can obtain salidroside through microbial synthesis, avoiding damage to the ecological environment and in line with the concept of sustainable development.
Usability: Most of the existing Rhodiola rosea related products are made from raw materials or crude extracts, which are inconvenient to use and have unstable functional components. Our products are made into standardized capsule dosage forms, each capsule independently packaged, which is convenient to carry and take with meals. Meanwhile, the content of salidroside in each capsule is fixed, ensuring the stability and consistency of the product's efficacy and making it easier for users to use.
Innovation: Subvert the traditional raw material restrictions. Traditional Rhodiola rosea products rely on wild picking, which is restricted by the plateau environment and scarce resources, resulting in high costs and unsustainability. We applied synthetic biology techniques to modify the metabolic pathways of Escherichia coli, transforming the extraction of salidroside from being dependent on natural conditions to being produced under microbial control. This not only breaks through the bottleneck of wild resources, but also can stably output high-purity components, reducing the raw material cost from a relatively high level to an acceptable one, and solving the core pain points of tight resources and high costs in the industry.
Demand: Design differentiated products based on the health needs and consumption habits of different age groups: Create "Youth Edition (Regular Edition) Capsules" for those aged 18 to 24 (suitable for refreshing and skin care needs, in line with the tastes of young people). Develop "Cardiovascular Care Gift boxes" for people aged 45 to 54 (meeting the essential needs of anti-altitude sickness and gift-giving, and conforming to the habits of offline channels). At the same time, it is further divided into "regular models" (focusing on core functions and offering high cost performance) and "high-end models" (adding other ingredients and providing exclusive health services), filling the gap in precise market segmentation and enabling people with different purchasing power and demands to match the products.(Figure 9)

We plan to launch two adaptive original products containing Salidroside within 24 months simultaneously - salidroside Capsules and salidroside Oral Liquid.
• Salidroside Capsules (200mg salidroside per capsule, 3g capsules, vegetarian capsules)
• Salidroside Oral Liquid (200mg salidroside per bottle, 30 bottles per box, vegetarian capsules)
Based on our HP investigation and analysis, we believe that our salidroside capsules and salidroside oral liquid are energy supplements specifically designed for urban people who pursue health and vitality. They are similar to energy drinks like Red Bull and Magic, but our products take salidroside as the core ingredient, which is a natural adaptogen found in traditional herbs. It has the functions of enhancing energy, boosting immunity and improving cognitive function. Our targ et market is those working professionals, fitness enthusiasts, students and anyone who hopes to improve their quality of life and seek natural health solutions in a fast-paced life. Whether it's oral liquid or capsules, our products are designed to replenish their energy in daily life and support cardiovascular wellness, thereby helping them maintain their best condition when facing challenges from work, study or sports. Our value proposition is to help users stay energetic and healthy in their busy lives by providing a natural and healthy way to enhance energy and endurance, while strengthening the immune system and supporting cognitive function. We believe that salidroside capsules and oral liquid can not only meet the health and vitality needs of urban elites, but also become an indispensable part of their daily lives.

• Profit model:Product sales. Targeting people who are concerned about cardiovascular and cerebrovascular health (such as middle-aged and elderly people, those who work at desks for long periods, etc.), salidroside health products (such as capsules, oral liquids, etc.) are launched and sold through e-commerce platforms, pharmacies, health product specialty stores and other channels.

• Cost control:Compared with traditional extraction methods, biosynthesis technology has a lower cost. For instance, at the current fermentation level of 10 grams per liter, the estimated comprehensive cost of biosynthesizing salidroside is 5,000 yuan per kilogram, which is much lower than the 30,000 yuan per kilogram for extraction from plants. By optimizing the production process, costs can be further reduced and profit margins can be increased.

• Customized services:based on consumers' health conditions, ages, living habits, etc., provide personalized salidroside health product customization plans, charge higher fees, and at the same time enhance customer stickiness and loyalty.

1. Middle-aged and elderly people: Usually over 50 years old, as they age, they become increasingly focused on maintaining cardiovascular health and proactive wellness. They have a strong demand for preventive products.

2. Office workers in a sub-healthy state: Those who stay up late for a long time, have high mental stress, lack exercise, and have irregular diets. Due to their lifestyle, this group often seeks support for maintaining vascular health and overall cardiovascular wellness.

3. Healthy people who pay attention to health preservation: They focus on daily health management and are willing to use natural extract products for preventive maintenance to reduce the risk of future illness.

4. Individuals focused on long-term vascular wellness: This group includes those with personal health background that motivates them to be particularly diligent in maintaining cardiovascular health through daily supplementation as part of a holistic approach to wellness.

Stage	Cycle	Milestone	Deliverables & Quality gate
1.Project initiation	W1~W2	Business case approval	Business plan, budget, core KPIs
2.Proof of Concept (POC)	W3~W10	Raw material bioequivalence & sensory testing	Business plan, budget, core KPIs
3.Formula locked	W11~W16	Final formula & Stability commitment	Capsules: Accelerated at 40°C/75%RH for 3 months, content ≥95%
4.Prototype & Preliminary test	W17~W24	Ten thousand capsules	Process SOP· The pilot batch inspection was qualified
5.Regulatory path initiation	W18~W30	China's "Health Food" dual regulation application	Capsule Registration (Function Claim: Relieve physical fatigue)
6.On-site testing	W25~W36	A 4-week clinical controlled trial involving 100 people	There were no serious adverse reactions
7.Supply chain lock-in	W31~W40	Secondary verification of contract manufacturers and raw materials	·Sign a framework contract for the next three years · Conduct a full COA inspection of raw materials
8.Verification of large-scale production	W41~W48	30,000 cans / 100,000 capsules engineering batch	The OEE of the production line is ≥75%, and the release qualification rate is ≥99%
9.Regulatory approval	W49~W75	Obtain the filing	Blue Hat filing/registration account
10.Preparation for listing	W75~W94	Listing license & Registration approval document	The packaging draft has passed the regulatory review
11.Listing & review	M25~M26	The first batch consists of 200,000 cans and 50,000 boxes	The repurchase rate target within 30 days of listing is ≥15%. · Project review report

• Internal: Functional Ingredient Laboratory (HPLC, GC-MS, Microbial Limit Room)

• External: National Research Center for Plateau Medicine

SGS (Stability, Heavy Metals, Pesticide Residues)

Category	Amount (RMB)	Explanation
Raw Materials and Inspection	3.2 million	Salidroside 99% raw material 40kg; Efficacy, stability, toxicology
Product Safety and Efficacy Studies	1.8 million	100-person RCT Ethics, statistics, CRO
Regulatory Declaration	1.2 million	Blue Hat Filing & Registration Validation of functional ingredients and methodology
Pilot production/mold	0.9 million	Beverage can necking molds, capsule filling machine models
Unforeseeable risk	0.5 million	Price/Regulatory mutation buffer
Total	7.6 million	The first 24-month budget

• Based on the ideas and aspects mentioned above, we have listed the following types of capital requirements:

Research and development expenses: 2 million yuan;
Pilot production expenses: 500,000 yuan;
Clinical trials: 5 million yuan;
Marketing promotion: 3 million yuan.

Position	Number of positions held	Main business	Ability
Project Manager	1~2	Be responsible for controlling the progress of the entire project and dispatching tasks to supervise the completion progress and quality of the tasks	Have excellent leadership skills and a broad perspective, and be able to strictly follow the time line
Formula scientist	8~12	Be responsible for research and development as well as completing professional research	Possessing solid professional knowledge and good execution ability
Clinical affairs	6	Be responsible for the clinical research during the observation and testing stage	Possess excellent analytical skills and be able to communicate in a timely manner
Registration regulations	4~8	Be responsible for registration and obtaining authorization from government agencies such as the Blue Hat	Communication skills and excellent ability to integrate materials
Supply chain quality inspection	≥10	Be responsible for long-term and regular supply chain operation to ensure that there is no fluctuation in quality	Willing to work outside for a long time and have the ability to report problems in a timely manner
Marketing Department	10~15	Responsible for marketing, supervising market development and conducting market research, etc	It requires an extremely strong ability to organize analysis reports and a deep understanding of the biopharmaceutical market
Marketing Department	20	Be responsible for visiting the target customer groups and merchants, establishing good relationships with them, and ensuring the profit situation both online and offline	Good communication skills are required and the ability to calculate and analyze market conditions is also necessary

Risk dimension	Specific context	Probability	Influence	Preventive and mitigation measures
Technology	Salidroside has a strong bitter taste and the concentrated capsules have a poor taste	Intermediate	High	Add sucrose or fruit juice sweetener ingredients to optimize the taste
Regulations	The national list of raw materials for health food has been adjusted	Low	High	Prepare the filing path for "nutritional food". Establish a six-month regulatory monitoring window
Funds	Clinical expenses overspent	Intermediate	Intermediate	• Bind stage payment nodes to CRO · Set 10% management storage • Bind stage payment nodes to CRO• Set up 10% management reserves • Bind stage payment nodes to CRO• Set up 10% management reserves • Bind stage payment nodes to CRO• Set up 10% management reserves • Bind stage payment nodes to CROSet a 10% management reserve
Supply chain	The raw materials for Rhodiola rosea on the plateau were poor	Intermediate	Intermediate	Sign up for 2 GAP bases in Xizang and 1 base in Yunnan• Establish a 12-month safety stock. • Bind the stage payment nodes to the CRO• Set up 10% management reserves • Bind stage payment nodes to CROSet a 10% management reserve
Market	Competing products rush to register "Red Hat”	High	Intermediate	• Submit the trademark and design patent six months in advance. • The packaging appearance is designed with differentiated coating materials. • Submit the trademark and design patent six months in advance • The packaging adopts a differentiated "space aluminum can" design. • Submit the trademark and design patent six months in advance • The packaging adopts a differentiated "space aluminum can" design. • Submit the trademark and design patent six months in advance • Packaging adoption • Submit the trademark and design patent six months in advance • The packaging adopts differentiated "space aluminum cans”
Reputation	The claim of efficacy has sparked anti-counterfeiting efforts	Intermediate	High	All claims are based on published RCTS; The tag adopts the filing language. The legal department establishes a 7x24 public opinion monitoring system

Financial Forecast:

• Projected sales: 22 million

• Gross Profit Margin: ≥45%

• Break-even Point: 10 million

Technical Indicators:

• 24-month shelf life content ≥ 90%

Business Indicators:

• Sales within 6 months of listing ≥ 25 million

• Gross profit margin ≥45%

• Field test completed (W36) : Efficacy/Safety GO/NO-GO

• Regulatory approval obtained (M22) : Market date frozen

1. Technical research and development capabilities:The team has members who are familiar with synthetic biology technology and can produce salidroside by modifying the metabolic pathways of Escherichia coli, completing the strain construction and metabolic pathway optimization in the laboratory small-scale trial stage.

2. Basic market analysis ability: Be capable of collecting market data of Rhodiola rosea products, preliminarily analyzing the demands of target customers (such as middle-aged and elderly people, white-collar workers), as well as the advantages and disadvantages of similar products.

3. Collaboration/Communication skills: Team members have clear divisions of labor, can cooperate to complete R&D, research and other tasks, and can also have initial communication with external parties to obtain industry information.

4. Large-scale production management capability: The current technology can only complete small-scale laboratory trials. In the future, it is necessary to master the intelligent operation of the fermentation workshop (such as parameter regulation of fermentation tanks, control of batch quality uniformity) and supply chain collaborative management (such as capacity planning, inventory turnover optimization), to ensure stable mass production from pilot-scale trials to hundreds of millions of grains per year, and avoid cost overruns or stockouts caused by production fluctuations.

5. International market operation capabilities: When expanding into overseas markets, it is necessary to possess skills such as cross-cultural communication (such as designing localized marketing scripts), international certifications (such as FDA and EU EFSA registration processes), cross-border logistics (such as overseas warehouse layout and tariff cost control), etc., to avoid market expansion being hindered due to water and soil incompatibility.

6. User data mining capability: When shifting from "selling products" to "health services", it is necessary to be able to build user profiles based on user consumption data (such as repurchase frequency and efficacy feedback), develop personalized recommendations (such as adjusting the dosage according to differences in physical constitution), achieve precise services tailored to each individual, and enhance user stickiness.

1. Industry technical experts: Engineers familiar with the large-scale production of Rhodiola rosea, helping to solve technical problems from the laboratory to pilot production and mass production (such as fermentation process optimization and cost control). It can solve the practical problems in large-scale production, making it more feasible to turn laboratory achievements into products and proving the reliability of the technical path.

2. Compliance and approval consultant: A professional who is familiar with the relevant regulations on the production and sale of food and health products, guiding the handling of production licenses, product filing and other procedures to ensure compliance. It can ensure that the project does not cross the legal red line, avoid delays in going public due to approval issues, and enhance the compliance and rigor of the plan.

3. Enterprise partners: Cooperate with suppliers of Rhodiola rosea raw materials, health product manufacturers (assisting in production), and sales channel partners (such as pharmacies and e-commerce platforms) to connect the entire chain from production to sales.The ability to form a complete industrial chain support indicates that the project is not only at the conceptual stage but also has actual resources and channels for implementation, making the business plan more convincing.

• Economic benefits: Adopting sustainable development: With the growing demand for highquality, sustainable health supplements, this project addresses a significant market need and has promising economic potential.

• Public health: The product will seek guidance from compliance advisors to ensure it does not violate the legal bottom line and avoid delays in market launch due to approval issues. At the same time, the intervention of technical experts and institutions makes the product more feasible and will not pose a threat to public health.

• At the ecological level: Reducing the exploitation of wild Rhodiola rosea can contribute to the ecological protection of the plateau, which is in line with the goal of coordinated development of "ecology and health" in "Healthy China 2030".

• At the industrial level: Promote the standardization of synthetic biology applications in the field of natural products to provide a model for the sustainable development of active ingredients from similar endangered plants.

• Unintended consequences: People are concerned about whether taking health supplements will have negative effects and whether they are truly effective.

• Trust building for risk management and responsible application measures: Jointly released the "White Paper on Synthetic Biohealth Components" with industry associations, publicly disclosing production processes and testing standards; Introduce a "30-day no-reason refund" policy to lower the threshold for consumers to try it. Seek out experts or well-known figures with high credibility in the local community to endorse and enhance external publicity.

• Product safety: Before the product is put on the market, list in detail the possible side effects or allergic symptoms.

• Technical risks (such as decreased strain stability) : Establish a strain backup library and conduct regular screening and optimization.

• Market risks (such as low-price impact from competing products) : Protect core technologies through patents and strengthen the differentiated selling points of "synthetic biology + stable efficacy".

• Compliance risks (such as changes in policy approval) : Hire a professional compliance team to follow up on policy developments in advance and reserve a buffer period to adjust plans.

Long-term roadmap (5-10 years)(Figure 10):

• Year 1 (Initial implementation) :

After completing the approval of food/health product licenses through customer channels, prioritize the launch of "small-scale pilot projects" : Cooperate with 2-3 office buildings in first-tier cities to introduce portable salidroside anti-fatigue packs specifically for white-collar workers.
Products are laid out in pharmacies in plateau areas (such as Qinghai and Xizang) to test market feedback.
Online, through platforms such as Xiaohongshu and Douyin, topics like "Anti-fatigue in the Workplace" and "Essential for high-altitude Travel" were used to invite users to share their usage experiences, initially establishing brand awareness. Objective: Cover 10 cities, accumulate 50,000 users, and verify the effectiveness of the channel.

• Year 2 (Expansion phase) :

Expand the scope of offline cooperation: Enter chain pharmacies and community health service centers, and launch "family packages" for middle-aged and elderly people, paired with health manuals.
Open an official flagship store online, optimize the logistics system, achieve nationwide delivery within 3 days, and keep the return rate within 5%.
Initiate user research, collect suggestions on dosage forms and flavors, and prepare for the next stage of product iteration.
Goal: Cover 50 cities and achieve an annual sales volume exceeding 10 million yuan.

• Year 3-4: Expand the product line and serve more people

Based on the feedback from the pilot program, two derivative products have been developed: "Fruit-flavored Chewable Tablets" suitable for children and "Energy Gel" for outdoor enthusiasts, etc.
Expand B-end cooperation: Collaborate with insurance companies to incorporate products into the "Health Management Gift Package"; In collaboration with the Plateau Tourism company, it is recommended as an essential travel item.
Objective: The user base will exceed 1 million, the product line will cover three core groups, and the annual sales will reach 50 million yuan.

• Years 5 to 7: Technological upgrading and going international

Upgrade the microbial synthesis technology to further reduce the production cost of salidroside by 40%, while enhancing its purity to meet international market standards.
Entering overseas markets: Focusing on the differentiated selling point of "natural ingredients + synthetic biotechnology", it has completed certification in countries such as Germany and Japan that attach great importance to the safety of health products.
Establish overseas warehousing centers to shorten international logistics time, with the goal of having the overseas market account for 30% of the total revenue.

• Years 8-10: Ecological expansion and transformation into a health service provider

Open the synthetic biology technology platform to provide technical support for the production of other natural ingredients (such as ginseng and Ganoderma lucidum), charge technology licensing fees, and form a "product + technology" dual revenue model.
Build a "health data platform" : Based on users' consumption data (such as anti-fatigue effects and changes in physical constitution), provide personalized health care plans, connect users with nutritionists and doctors, and shift from "selling products" to "providing health solutions".
Objective: To serve over 5 million users worldwide, become a benchmark enterprise in synthetic biology in the health field, and drive technological upgrades in the industry.