To enable the efficient heterologous synthesis and accurate detection of salidroside, we have developed a highly modular engineered platform. This system consists of three functionally independent and interchangeable modules, designed to streamline the optimization process and facilitate reusability in future studies:

1. Synthesis Module: This core module integrates the key enzymes, such as kivD and par, that catalyze the salidroside biosynthetic pathway for the de novo production of salidroside.

2. Enhancement Module: This module contains genetic elements engineered to enhance the supply of two key precursors—phydroxyphenylpyruvate and UDP-glucose—aiming to boost the overall metabolic flux and overcome production bottlenecks.

3. Detection Module: This module comprises a transcription factor-based biosensor capable of specific recognition and signal response to salidroside, enabling real-time, high-throughput screening of production yields.

Our project strictly adheres to a core principle of modularity. Building on the valuable work of previous iGEM teams, we have further refined our design, creating a system that is not only highly efficient but also exceptionally versatile. By simply replacing key components within the modules, this platform can be rapidly adapted to produce other high-value, structurally similar compounds.

We believe this design framework provides future iGEM teams with a validated, reliable solution and a library of key genetic parts. This represents not only a technical contribution but also embodies the spirit of collaboration and knowledge sharing championed by iGEM.