As a comprehensive university that has grown alongside the Special Economic Zone and enjoys high prestige both domestically and internationally, Shenzhen University is renowned for its exceptional research capabilities, innovative spirit, and remarkable "SZU Speed." We sincerely appreciate the comprehensive support provided by Shenzhen University for this project. Its state-of-the-art research facilities, abundant academic resources, and professional guidance from faculty members have created outstanding research conditions for us. Here, we express our profound gratitude for the top-tier academic environment offered by Shenzhen University.

The College of Life Sciences and Oceanography at Shenzhen University serves as a key base for scientific research and talent cultivation in the field of life sciences in South China, boasting a strong faculty and comprehensive research platforms. We sincerely appreciate the professional support provided by the school for this project. Its top-tier laboratory resources and profound academic expertise in cutting-edge fields such as life sciences and marine biology have endowed our project with a unique perspective and a high starting point. We are grateful for the experimental platforms and essential support generously offered by the school during the project, enabling us to carry out our work smoothly.

Due to technical challenges in laboratory aphid breeding, we contacted Professor Wang Jinjun's team at Southwest University. With their profound expertise in the pathogenic mechanisms of the brown citrus aphid, Professor Wang's team provided us with a batch of live aphid samples suitable for laboratory cultivation. Additionally, we engaged in in-depth discussions with the team regarding the collection and cultivation of live samples.

Dr. Lai Duo, Associate Researcher at the Guangdong Academy of Agricultural Sciences, is an expert in citrus fertilization and soil research. We obtained from him the modification method for Metarhizium and the engineered pBer vector.

During the protein extraction and detection process in E. coli with the transformed plasmid, we failed to observe the target protein band. Therefore, we consulted HZAU-China, which has extensive experience in this field, and they provided us with the relevant plasmid.

The discussion with BWYA centered on two projects: feline allergy treatment and citrus pest control, addressing common challenges such as experimental design, cost control, and delivery methods of RNA interference technology. This meeting provided us with a clearer understanding of the core challenges facing RNA interference technology in agricultural applications, including delivery methods, cost control, and regulatory compliance, while also offering direction for subsequent project optimization and translation.

To enhance the accuracy of our YOLOv-based visual recognition model and advance large-scale data transmission capabilities, we held extensive discussions with BIT-LLM, which possesses extensive experience in artificial intelligence and software development.

We collaborated with multiple iGEM teams worldwide to co-author a series of Instagram articles addressing the United Nations Sustainable Development Goals, successfully completing the "Goal 15: Life on Land" topic.

We engaged in a cross-border collaboration with Arizona State University on an educational project to create multilingual children's books adaptable to diverse cultural contexts, thereby promoting scientific knowledge among young audiences across different linguistic groups.

In collaboration with UAM, we shared our team's experiences during the iGEM competition, detailing challenges encountered, solutions implemented, and preventive measures taken, to draft a practical guide for future iGEM teams.

We collaborated with 33 iGEM teams, including CJUH-JLU-China, to co-author the popular science handbook "Debunking Synthetic Biology Myths," contributing to public awareness of synthetic biology scams and the advancement of this field.

We collaborated with six iGEM teams—including CSU, FUDA, SCAU, SUST, and ULINK—to conduct project exchanges. Through mutual Q&A sessions, we resolved outstanding issues within our respective projects and shared the outcomes of our iGEM initiatives with students from ULINK Education.

Focusing on the four fundamental areas of life—clothing, food, housing, and transportation—we collaborated with multiple iGEM teams to organize the "Essentials of Life" lecture series in Shenzhen's Pingshan Community. During the sessions, we specifically centered on the "food" dimension to educate the public on the advantages and disadvantages of conventional chemical pesticides versus RNA-based biopesticides. To enhance engagement, we used a self-designed interactive game to deepen participants' understanding of biopesticides.

We have taken into account the lack of conditions for synthetic biology research in some regional institutions. Leveraging our university’s "regional assistance" practical mission, we have established a collaboration with Han-Shan Normal University. We assist students at Han-Shan Normal University in mastering the theories of synthetic biology, engage in project discussions with them, and jointly complete related experiments.

SnapGene is a widely-used software in molecular biology, primarily employed for DNA sequence visualization, as well as the design, simulation, and documentation of molecular cloning experiments. It was developed by a team led by Professor Benjamin Glick at the University of Chicago to address the inefficiencies and incomplete record-keeping of traditional cloning workflows. We sincerely appreciate the software support and license provided by SnapGene for this project, which significantly enhanced our experimental efficiency and success rate.

GSAP (GreenSock Animation Platform) is a renowned professional-grade JavaScript animation library, celebrated for creating high-performance interactive experiences. We extend our sincere gratitude for its support in developing our project Wiki page, as its efficient and user-friendly animation capabilities significantly reduced the time required to build complex dynamic effects.

MathWorks stands as a global leader in engineering and scientific software. Its flagship product, MATLAB, leverages its core strength in matrix-based computation to integrate programming, numerical computing, visualization, and simulation into a high-performance interactive environment. For our project's hardware development, we are profoundly grateful for the convenience afforded by MATLAB's diverse toolboxes and extensive hardware support.

Autodesk is a world-leading provider of both 2D and 3D design, engineering, and entertainment software. The company specializes in digitally designing and modeling the world we live in, with its core business focused on delivering a suite of professional design applications. In our project, the exceptional 3D modeling and visualization capabilities of Autodesk software provided robust support for our design work, significantly enhancing both our efficiency and the professionalism of the final deliverables. For this, we express our sincere gratitude.