Activity: Test CTX antimicrobial activity against Penicillium digitatum and Geotrichum candidum.
Protocols: Microplate test of CTX against Penicillium digitatum and Geotrichum candidum.
Results: The CTX inhibited the growth of both fungi at 50 µM.
Period: 08.2025.
Scientists: Bruno Batista, Enrico Baeta Theodoro, Ana Paola Lopez Tarqui, Anna Luiza Graf Rocha, Maria Eduarda Tichauer da Rocha.
Activity: Test CTX antimicrobial activity against potential production hosts: Escherichia coli, Saccharomyces cerevisiae, Aspergillus oryzae.
Protocols: Microplate test of CTX against potential production hosts.
Results: CTX inhibited the growth of E. coli and A. oryzae, whereas yeast exhibited good resistance.
Period: 08.2025.
Scientists: Bruno Batista.
Activity: Test if CTX could reduce Candidatus Liberibacter asiaticus levels in contaminated leaves.
Protocols: Test of CTX against Candidatus Liberibacter asiaticus in leaves.
Results: The test was not effective in detecting a reduction in Candidatus Liberibacter asiaticus (Clas) population within the tested timeframe, even in the positive control.
Period: 08.2025 - 10.2025.
Scientists: Daniela Beatriz da Silva, Bruno Batista.
Activity: pIGEM001-sfGFP-CTX Construction and E.coli Transformation.
Protocols: PCR, NEBuilder, Miniprep, Bacteria transformation, Electrophoresis.
Results: We were able to build the pIGEM001 plasmid and transform it in E. coli bacteria.
Period: 08.2025.
Scientists: Bruno Batista, Maria Eduarda Tichauer da Rocha.
Activity: Expression and purification of sfGFP-CTX from E. coli using IMAC.
Protocols: Bacteria Expression, Chromatography purification, SDS-page.
Results: We were able to purify sfGFP-CTX from cultivation media through column chromatography.
Period: 08.2025.
Scientists: Ana Luiza Hernandes Sandano.
Activity: Cleavage and Isolation of CTX using TEV protease.
Protocols: CTX purification by TEV protease, CTX measurement by nanodrop.
Results: We were able to cleave CTX from sfGFP and measure it by nanodrop quantification.
Period: 08.2025 - 09.2025.
Scientists: Bruno Batista, Ana Luiza Hernandes Sandano.
Activity: pIGEM002-sfGFP-CTX construction.
Protocols: PCR, NEBuilder, Miniprep, DNA purification, Bacteria transformation, Electrophoresis.
Results: We were able to build pIGEM002-sfGFP-CTX and transform it in Aspergillus oryzae (ORY7).
Period: 08.2025.
Scientists: Bruno Batista, Maria Eduarda Tichauer da Rocha.
Activity: pIGEM002-sfGFP-CTX transformation in Aspergillus oryzae (ORY7).
Protocols: Digestion, Filamentous fungi protoplasting, Filamentous fungi transformation, Filamentous fungi DNA extraction, Electrophoresis, Protein quantification by Lowry assay, SDS-PAGE.
Results: We were able to build pIGEM002-sfGFP-CTX and transform it in Aspergillus oryzae (ORY7).
Period: 08.2025 - 09.2025.
Scientists: Bruno Batista.
Activity: pIGEM003-Aga1p-CTX construction and yeast transformation.
Protocols: PCR, Digestion, Ligation, Bacteria transformation, Yeast transformation, v5-Immunodetection.
Results: We were able to build pIGEM003-Aga1p-CTX and transform it in Saccharomyces cerevisiae.
Period: 09.2025.
Scientists: Bruno Batista, Davi de Castro Gomes.
Activity: Yeast cultivation and CTX isolation by chemical cleavage.
Protocols: Yeast cultivation, CTX purification by chemical cleavage, CTX measurement by nanodrop.
Results: We were able to detect CTX by nanodrop quantification in yeast expressing pIGEM003.
Period: 09.2025.
Scientists: Bruno Batista, Davi de Castro Gomes.
Activity: Integration of CTX at the C-terminus of amyABC genes in Aspergillus oryzae.
Protocols: Digestion, Filamentous fungi protoplasting, Filamentous fungi transformation, Filamentous fungi DNA extraction, Electrophoresis.
Results: We did not confirm any positive transformants from our transformation attempt.
Period: 09.2025 - 10.2025.
Scientists: Bruno Batista.
Activity: Solid-state fermentation using orange peel as substrate with Aspergillus oryzae.
Protocols: Solid-state fermentation using orange peel, Protein quantification by Lowry assay, SDS-page.
Results: We confirmed that Aspergillus oryzae can grow and produce high amounts of amylase using orange peel as a substrate in solid-state fermentation.
Period: 09.2025.
Scientists: Bruno Batista, Maria Eduarda Tichauer da Rocha, Anna Luiza Graf Rocha.