

Generation
Project Origins and Problem Statement
In recent years, the implementation of aesthetic education in China's basic education system has faced structural challenges. Art and music classes are often overshadowed by core subjects, and the shortage of teachers and facilities,especially in rural areas, further limits students' access to quality art education. As a result, art is frequently treated as a luxury rather than a necessity.
In response, we proposed the iRis project to explore how synthetic biology can offer a new approach to art education. Rather than replacing art with technology, iRis uses biotechnology to lower creative barriers and expand artistic expression. By developing a controllable biological pigment system, we aim to turn the laboratory into a creative space where children can “paint with life” and where art comes alive again — bridging synthetic biology and artistic creation through color.
Concerns: Safety and Societal Considerations
Throughout the design and implementation of the iRis project, we have prioritized biosafety, ethical responsibility, and social acceptance. While our goal is to make synthetic biology a new medium for art education, the educational context and the involvement of open public audiences require us to meet higher standards of safety and social responsibility.
(1) Biosafety Considerations
Click to see more ›Chassis selection: We use well-established non-pathogenic E. coli strains such as BL21 (DE3) and DH5α, which lack virulence factors and have low competitiveness in natural environments, making them unlikely to survive or reproduce outside the lab.
Waste Disposal: Our project properly handles bacteria-containing waste through methods such as autoclaving, which further eliminates potential risks with extremely low probability.
(2) Ethical Concerns and Public Acceptance
Click to see more ›Public perception of using synthetic biology as a tool for artistic expression inevitably involves cognitive and psychological concerns. To address these, we conducted interviews with art educators and public representatives to better understand their attitudes and incorporated their feedback into our project design. For example, we designed public acceptance surveys to explore perceptions of microbial art and concerns about hosting microbial art exhibitions. Additionally, through science outreach lectures, exhibitions, and open lab activities, we aimed to help the public understand the principles and significance of our project more intuitively, thus improving its social acceptability.
(3) Educational Ethics and Student Protection
Click to see more ›Given the involvement of minors in educational settings, we place particular emphasis on privacy protection, psychological safety, and ethical boundaries. In future implementation stages, we plan to collaborate with teachers and psychology experts to co-design age-appropriate, engaging, and inspiring educational activities. Our goal is to ensure that students experience a safe, positive, and enriching journey as they explore the intersection of science and art.
Feasibility Analysis
iRis is not just a proof of concept confined to the laboratory — it is designed to extend into education, art, and public cultural engagement, transforming from a bench-top experiment into a scalable and replicable educational and creative tool.
To better analyze its feasibility, we divide this section into two key parts: Stakeholder Interaction and Responsibility Indicators.
Stakeholder Interaction
The stakeholders of iRis form a “petal-like” layered structure: with the project itself at the center, relationships expand outward in multiple layers. From core creators to the general public, each layer plays a distinct role in shaping the design, execution, and dissemination of the project. By analyzing the interests, influence, and interaction models of each layer, we can refine our strategies and better achieve the goals of education, art, and science communication.

Core Layer — iRis
Click to see more ›Interest:
To realize the cross-disciplinary innovation of synthetic biology × art education, exploring microbial art as a tool for creative expression and teaching.
Influence / Relationship:
As the project itself, iRis determines the technical route, educational framework, and public engagement strategies, forming the nucleus of the stakeholder network.
Interaction / Optimization:
Through continuous iteration in experimental design, artistic creation, and educational activities, iRis enhances both its scientific feasibility and artistic expressiveness while integrating feedback from all stakeholder layers.
First Layer — Bioartists & Museums
Click to see more ›Interest:
Direct participation in art creation, exhibition curation, and science communication activities, with a focus on interdisciplinary innovation and public art experiences.
Influence / Relationship:
They provide creative guidance, technical support, and exhibition platforms — museums offer venues and promotion channels, while bioartists bring expertise in aesthetics and interactive design.
Interaction / Optimization:
Collaborative Creation: Co-design exhibitions and workshops that integrate scientific experiments with artistic works.
Audience Feedback Integration: Gather visitor feedback to improve the interactive experience of the light-controlled microbial “brush.”
Training & Knowledge Transfer: Share methods for integrating science and art, promoting interdisciplinary education practices.
Second Layer — Art Organizations, Project Collaborators, Professors
Click to see more ›Interest:
Focus on interdisciplinary education, innovative curriculum development, and science-art educational practices, seeking to explore new teaching approaches and research directions through the project.
Influence / Relationship:
Provide resource support, academic guidance, and outreach channels, influencing the feasibility and impact of iRis in education and research fields.
Interaction / Optimization:
Curriculum Integration: Incorporate iRis into course modules, such as synthetic biology experiments in biology classes or color theory in art classes.
Workshops & Seminars: Co-organize lectures, lab sessions, and creative camps to deepen students' understanding of the science–art nexus.
Expert Review: Academic experts evaluate experimental design and educational plans to ensure scientific rigor and pedagogical effectiveness.
Third Layer — Specialists in Application Areas / SynBio Institutes & Researchers
Click to see more ›Interest:
Interested in the technical implementation, biosafety, biosynthetic pathways, and scalability of iRis, with a focus on its application value in educational and artistic contexts.
Influence / Relationship:
Provide technical evaluation, experimental optimization, and research support, influencing the reliability and sustainable development of iRis.
Interaction / Optimization:
Technical Consultation: Offer guidance on light control, pigment synthesis, and system optimization.
Safety & Compliance: Ensure that the project meets biosafety standards and can be safely implemented in educational and exhibition settings.
Research Collaboration: Partner with research institutes to explore the potential of iRis in education and bioart expansion, facilitating the translation of scientific results.
Outer Layer — Public
Click to see more ›Interest:
Curious about life sciences, creative expression, and interactive experiences, with the potential to actively participate in educational programs, exhibitions, and outreach activities.
Influence / Relationship:
Although their direct impact on technical decisions or curriculum design is limited, public engagement and feedback are essential for project promotion, social acceptance, and the dissemination of art education concepts.
Interaction / Optimization:
Community Engagement: Attract public participation through exhibitions, open lab classes, and creative workshops.
Feedback Collection: Gather opinions on interaction, aesthetics, and educational value to refine project design.
Awareness & Education: Enhance public understanding of the intersection between synthetic biology and art, advancing scientific literacy and art education outreach.
Responsibility Indicator
Through the Triple Bottom Line (People, Planet, and Progress in iGEM) framework, we evaluated the social, environmental, and technological impacts of the iRis project.
People – Social Impact
Positive Impacts
Community Engagement: Students and the public can participate in bio-art activities through experiments and creation, strengthening their interest and engagement in both science and art.
Education and Awareness: The project enhances aesthetic education and scientific literacy, helping young learners understand the interdisciplinary value of art and life sciences.
Project Adjustment: By collecting feedback from students, teachers, and the public, we continuously refine our curriculum and workflows to ensure the project meets real educational and societal needs.
Negative Impacts
Public Concerns: Some members of the public may still have doubts about synthetic biology, requiring additional outreach and safety communication.
Limited Resources: Activities may not reach all target groups, especially in resource-limited rural areas.
Safety Concerns: Handling living microorganisms requires strict adherence to biosafety protocols to prevent potential risks.
Planet – Environmental Impact
Positive Impacts
Reduced Pollution: iRis avoids the pollution associated with traditional chemical pigment production. Chemical indigo synthesis uses toxic reagents such as aniline and nitrobenzene, whereas our bio-pigments are derived from tryptophan in E. coli and require only natural media components like glucose and yeast extract, producing no toxic wastewater.
Low-Energy Production: While chemical pigment production relies on high-temperature, high-pressure reactions and multiple purification steps, our system operates at 37 °C under atmospheric pressure and requires only centrifugation, significantly reducing carbon emissions.
Negative Impacts
Low-Probability Risk of Leakage: Although we use attenuated chassis strains, vigilance is required to prevent possible escape or horizontal gene transfer.
Unknown Long-Term Effects: The long-term ecological impact of synthetic biology in natural environments remains uncertain and requires continuous study.
Progress in iGEM – Community Impact
Positive Impacts
New Interdisciplinary Paradigm: iRis expands the traditional focus of iGEM projects beyond medicine and environment by introducing art education as a new application scenario, offering fresh perspectives for interdisciplinary integration.
Team Collaboration: The project fosters collaboration with other iGEM teams and industry partners, strengthening cross-disciplinary cooperation and promoting knowledge sharing and technical advancement.
Knowledge Contribution: Our project introduces new part designs and innovative solutions that enrich the iGEM knowledge base.
Negative Impacts
High Technical Complexity: The RGB light-control system of iRis involves multi-plasmid co-expression and requires optimization of plasmid compatibility, posing technical challenges.
Market Uncertainty: Despite its innovation, the market potential and public acceptance of such bio-art applications still need further validation.
Field Study
To gain a deeper understanding of the real-world needs behind the fusion of art and science — and to explore the feasibility and potential of the iRis project within authentic artistic contexts — we conducted a field study in Dafen Oil Painting Village in Shenzhen. There, we held in-depth interviews with Zhao Xiaoyong and Zhou Yongjiu, the two central painters featured in the acclaimed documentary China’s Van Goghs. Their artistic journeys, creative philosophies, and reflections on the intersection of art and science offered us invaluable insights that continue to shape our project.
Zhao Xiaoyong: From Replication to Creation
Zhao Xiaoyong, one of the most representative artists in China’s Van Goghs, has spent nearly three decades reproducing Van Gogh’s works in Dafen. Beginning his career as a copyist painting for commercial orders, Zhao gradually shifted toward original creation, drawing inspiration from his daily life and surroundings. Entirely self-taught and refined through years of practice, he honed his techniques and sensitivity to color. A pivotal moment came in 2018, when he saw Van Gogh’s original paintings in Europe — an experience that prompted his decisive transition to original work and deepened his conviction that “everything in the world can become a subject of painting.”
During our conversation, Zhao shared his profound understanding of Van Gogh and IRises, as well as the lessons learned from thirty years of artistic practice. He emphasized that Van Gogh’s works are imbued with a love for life and a sharp sensitivity to nature — whether iRises or sunflowers, they all symbolize vitality, which he believes lies at the very heart of art itself.
Discussing our concept of recreating IRises using three bacterial pigments — purple, blue, and green, Zhao offered concrete suggestions. He noted that focusing on the main color tones would be enough to capture the painting’s essence without reproducing every detail. The three cool colors, he suggested, could represent the petals, sky, and leaves, while the buds and flower centers could be simplified using shades of blue and white. Acknowledging that bacterial pigments lack the textured thickness of oil paint and that silk as a substrate is inherently flat, Zhao encouraged us to experiment with methods to enhance three-dimensionality of IRises.
Zhao also reflected on the evolving relationship between art and science. Contemporary art, he observed, is constantly experimenting with new materials and forms of expression. While technology can produce visual effects beyond the reach of traditional media, true innovation ultimately stems from the essence of the artwork itself — it is not the technique but the power of the work that moves people. He also discussed how Dafen Oil Painting Village has lowered the threshold for public engagement with art, providing subtle aesthetic experiences to ordinary audiences. He advised us to adopt a similar approach by designing interactive, participatory experiences that invite viewers to “ come closer first, and then understand. ”
"The core of painting is free expression, and there has never been a fixed answer to how it should be presented."

Zhou Yongjiu: Art, Emotion, and Engagement
Zhou Yongjiu, another main figure in China’s Van Goghs, also began his career producing replicas in Dafen. After encountering Van Gogh’s work by chance in 1994, he started copying his paintings and, following the 2008 financial cRisis, shifted toward original creation. Free from dependence on orders, Zhou began producing works that more fully expressed his own emotions and reflections. Deeply inspired by nature and everyday life, his art explores the relationship between humans and nature, as well as the emotional significance of art in daily existence.
In our interview, Zhou shared his distinctive interpretation of Van Gogh and IRises. He believes that Van Gogh expressed emotion through vibrant colors, with the white iRises often appearing in the brightest areas of the canvas. Zhou emphasized that Van Gogh consistently focused on themes of nature and ordinary life, and that this concern endowed his work with emotional depth.
Regarding our idea of recreating IRises on silk using bacterial pigments, Zhou proposed several practical suggestions: enriching the color layers by blending the three pigments, altering the texture through freeze–thaw or heat treatments, and designing subtle surface textures on the silk to mimic the brushstrokes of oil paint. His advice offered valuable directions for both our future experiments and exhibition design.
On the topic of public engagement with art, Zhou noted that while people’s openness to art is increasing, the paths to understanding vary widely. A successful exhibition, he argued, should first entice people to pause and look, then gradually guide them toward deeper understanding. He emphasized the importance of public education and scientific communication in reducing unfamiliarity and hesitation toward microbial art.
Zhou is also actively involved in public art education, teaching painting to autistic and hearing-impaired students in a charity school. Through his “ show first, then encourage personal expression ” approach, he explores art’s therapeutic potential, helping students discover ways to express their inner worlds. This inspired us to consider the future applications of the iRis project in education and art-based therapy.

Insights for iRis
Through this field study, we not only deepened our understanding of the artistic language behind IRises but also gained valuable insights into color presentation, material processing, and public engagement. Both Zhao Xiaoyong and Zhou Yongjiu’s experiences demonstrate that the essence of artistic creation does not lie in technique alone but in the vitality, emotion, and thought that underpin it.
This philosophy resonates strongly with the original vision of the iRis project — to use synthetic biology to lower the barriers to artistic creation, bring art back into the fabric of everyday life, and make life itself the brush with which we paint.
Technical Road Map



Future
What we will do
While we have accomplished many of our initial goals, there remain areas with great potential for further exploration and refinement. Looking ahead, we have outlined several directions to continue improving and expanding our project. The following plans represent how we aim to build upon our current work and bring it to a more comprehensive level in the future.
Lack of conversation
The government:
At the very first of this aspect, we plan to initiate dialogue with relevant local and national departments in China, such as the Zhuhai Environmental Protection and ecology Association and the National Center for Biotechnology Development. Our goal is to:
a.Present a comprehensive biosafety dossier detailing the containment measures and environmental Risk assessment of our engineered strains.
b.Explore pathways for policy advocacy, contributing to the development of clear regulatory frameworks for bio-art materials and their commercial application.
c.Seek guidance on scaling our technology from the laboratory to controlled industrial or community settings.
Industry Partners:
To translate our proof-of-concept into tangible products, we will proactively reach out to potential industry partners.
a. Textile and Fashion Companies: Collaborate with sustainable brands to test our microbial dyes on a pilot scale, assessing durability, cost-effectiveness, and consumer acceptance.
b. Medical and Therapeutic Institutions: Establish partnerships with hospitals and rehabilitation centers to design and conduct clinical trials for our art therapy protocols, ensuring they meet ethical and efficacy standards.
Furture application
Prospects of Dye Applications
Click to see more ›The indigo and its derivatives synthesized through synthetic biology avoid the carcinogenic pollutants, such as aniline, commonly used in traditional chemical synthesis, thereby promoting sustainability. In this project, the three target dyes—indigo, Tyrian purple, and 4,4'-dinitroindigo—hold broad potential applications across multiple fields:
a. Textile Dyeing:Indigo and Tyrian purple, as classic dyes, demonstrate excellent performance in textile coloration. Although the microbial production pathway of 4,4'-dinitroindigo has not yet been fully established, its strong coloring and stability indicate significant potential as a textile dye.
b. Functional Materials:Tyrian purple, with its stacked structure and repeating conjugated π-bonds, can serve as a functional coating in applications such as dye-sensitized solar cells and conductive materials.
c. Antimicrobial Agents:Studies show that introducing position-specific nitro or halogen groups to natural products can significantly modify their physical properties and biological activity. Brominated indigo and 4,4'-dinitroindigo exhibit antibacterial properties, suggesting potential for further drug development.
Optogenetics Applications
Click to see more ›In this project, we employed an RGB light-sensitive system to spatially and morphologically control dye synthesis. Light serves as a reversible, low-cost inducer, enabling precise regulation of specific cells or locations within an organism. This approach offers a balance of cost-efficiency, safety, and simplicity, making it a practical synthetic biology strategy. Currently, optogenetic pathways are applied in areas such as neural modulation and targeted drug delivery, providing a platform for exploring fine-scale biological regulation.
Art Therapy
Click to see more ›We plan to collaborate with related professionals after our work has been completed. Before that, we learned about some significant information and considerations through interviewing. It is possibly accessible for us to complete in the following ways:
a. Co-develop standardized therapeutic toolkits with certified art therapists and psychologists. These kits will include tailored protocols for using our light-controlled dyes with specific populations.
b. Train practitioners on the safe and effective use of our bio-art tools in therapeutic settings.
Sustainable Fashion
Click to see more ›a. Community Workshops: Establish a social enterprise model where community members can learn the skills to produce and use these dyes, fostering local economic development centered on sustainability.
b. Creative application in sustainable fashion fields: Encourage collaboration between local artisans and sustainable fashion designers to create unique, eco-friendly clothing lines.
Bacterial Art Legal and Ethical Framework
Click to see more ›Pioneering Regulatory Guidelines: Propose a collaborative framework with legal experts, bioethicists, and microbiologists to address novel legal considerations in bacterial art, including biocontainment standards for public exhibitions, and liability protocols for unintended ecological impact.
What Remains to be Solved
Basal Leakage in the Light-Control System: Current Status: A slight basal expression occurs in the dark, causing a faint color haze in the background. Although the expression level is low, it reduces the sharpness of the patterns during painting.
Low Pigment Yield Due to Limited Multi-Enzyme Synergy: Current Status: The yield of purple pigment is significantly lower than that of indigo, which limits color saturation and fails to meet artistic creation needs.
Lack of a Quantitative Correlation Between Light Signals and Pigment Colors:Current Status: Currently, only the presence or absence of light stimulation can be used to control pigment production, without fine-tuning the color depth (e.g., light purple, medium purple, dark purple). A quantitative model linking light intensity, exposure time, pigment concentration, and visual color has not yet been established.