| BBa_25RIK1VS |
EcTnaA |
A gene from Escherichia coli encoding a kind of tryptophanase. |
| BBa_251L4NGE |
MaFMO |
A gene from Methylophaga aminisulfidivorans encoding flavin-dependent monooxygenase. |
| BBa_25UDOH90 |
YF1 |
The YF1 gene encodes a fusion histidine kinase. |
| BBa_25SQZ12O |
FixJ |
The gene fixJ encodes a protein that can be phosphorylated by YF1 to initiate downstream gene expression. |
| BBa_K2296017 |
Pfix-K2 |
This promoter can be activated by the phosphorylated protein FixJ, initiating downstream gene expression. |
| BBa_K1725040 |
PhlF |
The gene PhlF encodes a protein that inhibits the promoter pPhlF. |
| BBa_K1725000 |
pPhlF |
When the promoter pPhlF binds to the PhlF protein, its function is inhibited and downstream genes cannot be expressed. |
| BBa_25MREMGW |
PadC4 |
The gene encodes a di-guanylate cyclase that catalyzes the synthesis of c-di-GMP from GTP in a near-infrared light dependent manner. |
| BBa_25KQ28VU |
BphO |
The gene encodes a heme oxygenase that catalyzes the oxidation of heme to produce biliverdin,an essential cofactor that functions as a chromophore for light-sensing proteins. |
| BBa_256M7X59 |
YhjH |
A gene from Escherichia coli encoding a c-di-GMP-specific phosphodiesterase, involved in c-di-GMP degradation and maintenance of low intracellular c-di-GMP levels. |
| BBa_25S48UIO |
MrkH |
A gene from Klebsiella quasipneumoniae encoding a c-di-GMP-specific phosphodiesterase,which is a transcriptional activator that specifically responds to c-di-GMP and activates transcription from the synthetic promoter PmrkA. |
| BBa_K5210015 |
PmrkA |
A promoter from red light-responsive system,which is regulated by the MrkH protein, directly activates transcription from the mrkA promoter to initiates downstream gene expression. |
| BBa_250ASSOU |
SttH |
The gene encodes a flavin-dependent tryptophan 6-halogenase that relies on FAD and molecular oxygen as cofactors, and functions as a key enzyme in the biosynthesis of halogenated indole compounds. |
| BBa_K3722004 |
Fre |
A gene from Escherichia coli encoding a flavin reductase that primarily functions to continuously supply the flavin-dependent halogenase SttH with essential reduced cofactors, promoting the bromination reaction. |
| BBa_259FDUZF |
L3 linker |
The gene encodes an extended flexible peptide linker that covalently connects the two functional protein domains Fre and SttH in a fused configuration. |
| BBa_25WVGYOO |
pJ23119 |
pJ23119 is a strong constitutive promoter, capable of driving downstream gene expression independently of external regulation. |
| BBa_25MEK6RV |
ho1 |
The gene ho1 encodes the HO1 protein.HO1 protein is heme oxygenase, which catalyzes the conversion of heme to biliverdin. |
| BBa_25F1AWKV |
pcyA |
The gene pcyA encodes the PcyA protein.The PcyA protein is ferredoxin oxidoreductase, which catalyzes the conversion of biliverdin to phycocyanin. |
| BBa_25N1634Z |
mini-CcaS |
The gene mini-CcaS encodes the protein mini-CcaS.Removing two PAS domains and fusing together the GAF and HK domains with a truncated linker region, to form “miniaturized CcaSs”. Compared with wild-type, the miniaturized CcaSs is able to tightly regulate gene expression, achieving high expression of the target gene under inductive conditions and strict gene repression under non-inductive condition. |
| BBa_25JE4V2X |
CcaR |
The gene CcaR encodes the protein CcaR.It is a typical response regulator of the OmpR class, which consists of an N-terminal receiver domain and a C-terminal DNA-binding domain. |
| BBa_25C6K297 |
txtE |
The gene txtE encodes TxtE protein.TxtE protein is a novel cytochrome P450 enzyme.It can catalyze regiospecific 4-nitration of L-tryptophan.It can nitrate non-natural substrates that are structurally similar to L-tryptophan with decorated indole side chains and modified amino acid moieties. |
| BBa_25I36CTL |
txtD |
The gene txtD encodes TxtD protein.TxtD protein is a nitric oxide synthase (NOS) that generates NO from L-arginine. |
| BBa_25JSNLBP |
Fdr |
The gene Fdr encodes the protein FdR.FdR protein is a ferredoxin reductase that contains a flavin adenine dinucleotide (FAD) cofactor.It can reduce Fdx. |
| BBa_252BZZLO |
Fdx |
The gene Fdx encodes Fdx protein.Fdx protein is a small iron-sulfur protein ferredoxin.It can be reduced by the FdR protein. |
| BBa_K2012015 |
PcpcG2-172 |
PcpcG2-172 is a green light-inducible promoter. CcaS regulates the phosphorylation of CcaR by transferring the phosphate group (P) to CcaR; the phosphorylated CcaR then binds to the promoter PcpcG2-172, thereby further promoting the expression of the target gene. |
| BBa_252VLWW7 |
terminator DT25 |
This is a terminator, specifically the DT25 terminator derived from the pJFR2 plasmid constructed in the RGB system, which is used in conjunction with the promoter pPhlF. |
| BBa_J23100 |
pJ23100 |
pJ23100 is a strong constitutive promoter, capable of driving downstream gene expression independently of external regulation. |
| BBa_J23106 |
pJ23106 |
pJ23106 is a strong constitutive promoter, capable of driving downstream gene expression independently of external regulation. |
