Contribution

We focused on creating helpful resources, from fun educational projects and community events to easy-to-use tools and genetic designs, to make science easier to understand and inspire others to explore biology.

Contribution
All materials shared on this page are available under the Creative Commons Attribution 4.0 International (CC BY 4.0) license. You are free to share and adapt them with proper attribution.

Our Contribution: Educational Material

Our team created educational content, slide decks and hosted engaging events designed to support our iGEM team’s community outreach and engage young students with basic and relevant scientific topics. We also collaborated with the NIS Kazakhstan iGEM team to create a fairytale book, which teaches children about skin conditions, normalizing the occurrence of acne to help them feel more comfortable in their own skin. These contributions were motivated by the challenges we faced during our own project, and we wanted to make it easier for future teams to navigate similar hurdles.

Why It's Useful

The informational slide decks are an efficient, easy to follow tool that helped us explain complex subjects by breaking them down by topic. They also follow a slide deck format also allows us to use GIFs, videos, and graphics to present a visual representation of the topics. By incorporating various mediums into the presentations, we were able to make it simple for the students to understand concepts of DNA, the immune system, and inflammation better. Other educational material we developed includes the fairy tale storybook translated into multiple languages that makes its way to children to explain topics in a fun, engaging way.

Acknowledgements

Thank you to the NIS Kazakhstan team for introducing this collaboration opportunity.

Where to Access It

English Version: Link

Kazakh Version: Link

How to Use It

  • Access: Download our slide decks and fairy tale book from Canva.
  • Adapt: Customize the content to match your project theme or targeted age group.
  • Share: Use the materials in school workshops, community events, or online sessions to make biology fun and engaging.
  • Expand: Feel free to translate or remix the storybook to educate on other science or health topics, or co-create new versions with other teams to reach broader audiences.

Workshop Slide Decks



Our Contribution: Cell Identifier Model

Our team developed an open-source web-app tool that automatically detects and quantifies GFP expression (fluorescence) based on sample images of bacterial cells. We made this to address the challenge in biology of manually counting and measuring fluorescence of cells, which is time-consuming, subjective and inconsistent. The web-app we developed gives automated and reproducible quantification that any team may use for validation of their biosensor and expression analysis.

Key Features

  • Synthetic Cell Image Generation: Generates sample images of fluorescent bacteria given user input. Users are able to customize number of cells, cell morphology (pill-shaped E. coli or elliptical), and overlap of cells.
  • Automated Cell Detection: Uses a simple and classical computer vision with adjustable parameters: Gaussian blur for noise reduction, intensity thresholding for segmentation, morphological operations to separate touching cells, and area filtering to remove debris and artifacts.
  • GFP Expression Classification: Each detected cell is then classified by mean fluorescence intensity: Low brightness (< 73), Medium brightness (73-80), and High brightness (> 80).
  • Web-Based Interface: The app runs as a Flask application that can be used in a web browser. Users can upload images, adjust detection parameters with sliders, and download annotated results with cell counts, intensities, and brightness distributions. It may be accessed at https://cell-identifier-ij23.onrender.com/.

Applications for Other Teams

  • Biosensor validation: Quantify dose-response curves and logic gate behavior.
  • Promoter characterization: Compare expression levels between constructs.
  • Quality control: Monitor transformation efficiency and plasmid stability.
  • Time-series studies: Track fluorescence changes over time.

The modular code allows easy customization for different organisms, fluorescent proteins, or multi-channel imaging.

Our Contribution: Custom Genetic Constructs

We designed our own gene constructs for detecting NO and H₂O₂ and producing GFP as a reporter. These genes weren’t submitted to the Registry, but we fully designed and modeled them, and planned experiments to test them as part of our project. They were a key part of our system and show the core work we did in engineering and planning the biosensor-response system. The PDFs below contain annotated sequences of the genes, plus the EcoR1 and PstI restriction sites we needed for the restriction digest. Other teams or researchers can use these designs as a reference for building similar biosensor systems or for educational purposes. They are available under a Creative Commons Attribution 4.0 license, so you’re welcome to adapt, share, or build on them with attribution.