During our laboratory work, we developed and refined protocols to optimize outcomes. Through multiple iterations, we enhanced these protocols to ensure consistent and reliable results. This page compiles our finalized protocols for your reference, designed to support and streamline your laboratory troubleshooting.
This workflow outlines the cloning of complete plasmids into Escherichia coli . A separate protocol for Pseudomonas putida electroporation and transformation is available below.
Cloning of complete plasmids into Escherichia coli