Experiments

General Molecular Biology

Chemical Transformation

This protocol was created by Dr. Manish Kushwaha at the Cellular Computing Team at INRAE, and provided to us by our iGEM mentor Shreyas Samir Parkhie.

Competent Cells Preparation

This protocol was created by Dr. Manish Kushwaha at the Cellular Computing Team at INRAE, and provided to us by our iGEM mentor Shreyas Samir Parkhie.

Gel Electrophoresis

Glycerol Stocks

PCR

Reverse Transcription

S. pasteurii Mineralization

Calcium Mineralizing Media Recipes

This protocol is from the Hernandez Research Group at UCSF and was provided to us by our secondary PI Dr. Christopher Hernandez as well as Dr. Kelsey Defrates, a postdoctoral researcher at UCSF.

Media Recipes

This protocol is from the Hernandez Research Group at UCSF and was provided to us by our secondary PI Dr. Christopher Hernandez as well as Dr. Kelsey Defrates, a postdoctoral researcher at UCSF.

Mineralization with S. pasteurii

S. pasteurii Culture Preparation

This protocol is from the Hernandez Research Group at UCSF and was provided to us by our secondary PI Dr. Christopher Hernandez as well as Dr. Kelsey Defrates, a postdoctoral researcher at UCSF.

Manufacturing PDMS Channel-Pore Microfluidic Devices

This protocol is from the Hernandez Research Group at UCSF and was provided to us by our secondary PI Dr. Christopher Hernandez as well as Dr. Kelsey Defrates, a postdoctoral researcher at UCSF.

Sterilization

Protein Expression / Function Assays

Carbonic Anhydrase Assay

Curli Assay

Phenol Red Assay