Strain 1: Acetate Production
| Part Number | Part Type | Description | Organism |
|---|---|---|---|
| BBa_25U5FSLK | Basic | rhc_cbbL - Rhodobacter capsulatus Form I RuBisCO large subunit (Uniprot: O32740 · RBL1_RHOCB) | Rhodobacter capsulatus |
| BBa_25SSLHRH | Basic | psp_rbcX - rhc_cbbL - Picosynechococcus sp. RuBisCO chaperone RbcX (Uniprot: Q44177 · RBCX_PICP2) | Picosynechococcus sp. |
| BBa_25HYN9E1 | Basic | rhc_cbbS - Rhodobacter capsulatus Ribulose bisphosphate carboxylase small subunit (Uniprot: O32741 · RBS_RHOCA) | Rhodobacter capsulatus |
| BBa_25P60DXB | Composite | T7-lacO-rhc_cbbL | Rhodobacter capsulatus |
| BBa_25CQVB2G | Composite | psp_rbcX - rhc_cbbS bicistronic gene fusion | Picosynechococcus sp. / Rhodobacter capsulatus |
| BBa_25UC5AZ8 | Composite | RuBisCO/RuBisCO Chaperone Expression Cassette (rhc_cbbL–1C3 and psp_rbcX–rhc_cbbS–1C3) | Picosynechococcus sp. / Rhodobacter capsulatus |
| BBa_25UC5AZ8 | Composite | dci_bCA - Dolichospermum circinale β-Carbonic anhydrase (Uniprot: A0A0C1N7A1 · A0A0C1N7A1_9CYAN) | Dolichospermum circinale |
| Basic | csp_prkA - Cereibacter sphaeroides Phosphoribulokinase 1 (Uniprot: P12033 · KPPR1_CERSP) | Cereibacter sphaeroides | |
| BBa_2565FRU9 | Composite | T7-lacO-csp_prkA | Cereibacter sphaeroides |
| BBa_25UC9VHX | Basic | dci_bCA - Dolichospermum circinale β-Carbonic anhydrase (Uniprot: A0A0C1N7A1 · A0A0C1N7A1_9CYAN) | Dolichospermum circinale |
| BBa_2529E8AV | Composite | dci_bCA–rrnB T1 + T7Te | Dolichospermum circinale |
| BBa_25DY9B4B | Composite | prkA – bCA Expression Cassette | Cereibacter sphaeroides / Dolichospermum circinale |
| BBa_253J5UV1 | Composite | CBB+ Expression Cassette (rhc_cbbL–psp_rbcX–rhc_cbbS–csp_prkA–dci_bCA) | Picosynechococcus sp. / Rhodobacter capsulatus / Cereibacter sphaeroides / Dolichospermum circinale |
| BBa_251NB66M | Basic | psp_FDH - Pseudomonas sp. NAD+-dependent Formate Dehydrogenase (Uniprot: P33160 · FDH_PSESR) | Pseudomonas sp. |
| BBa_25AI44J9 | Composite | psp_FDH Expression Cassette | Pseudomonas sp. |
| BBa_25QFF6C9 | Basic | ckl_pta - Clostridium Kluyveri Phosphate acetyltransferase (Uniprot: A5N801 · A5N801_CLOK5) | Clostridium Kluyveri |
| BBa_25VBQ14X | Basic | bsu_ackA - Bacillus subtilis Acetate Kinase (Uniprot: P37877 · ACKA_BACSU) | Bacillus subtilis |
| BBa_25UCZPC9 | Composite | pta–ackA Expression Cassette | Clostridium Kluyveri / Bacillus subtilis |
Strain 2: PHBV Production
| Part Number | Part Type | Description | Organism |
|---|---|---|---|
| BBa_2506EXU2 | Basic | ssp_phaB - Synechocystis sp. acetoacetyl-CoA reductase (Uniprot: P73826 · PHAB_SYNY3) | Synechocystis sp. |
| BBa_25W1P5SZ | Composite | CRPbox_T7-phaB (Synechocystis sp.) | Synechocystis sp. |
| BBa_25CRIMKQ | Basic | avi_phaC - Allochromatium vinosum Poly(3-hydroxyalkanoate) polymerase subunit PhaC (Uniprot: P45370 · PHAC_ALLVD) | Allochromatium vinosum |
| BBa_25XH4PJ9 | Basic | hme_bktB - Haloferax mediterranei β-ketothiolase | Haloferax mediterranei |
| BBa_25JX6WGR | Composite | pPHBV Expression Cassette (T7–lacO–ssp_phaB–avi_phaC–hme_bktB) | Synechocystis sp. / Allochromatium vinosum / Haloferax mediterranei |
| BBa_25X3FQRD | Composite | pPHBV+ Expression Cassette (T7–lacO–ssp_phaB–avi_phaC–hme_bktB) | Synechocystis sp. / Allochromatium vinosum / Haloferax mediterranei |
| BBa_25GB84GD | Basic | sfl_tdcB - Shigella flexneri L-threonine dehydratase catabolic TdcB (Uniprot: P0AGF9 · TDCB_SHIFL) | Value |
| BBa_25AOBJBZ | Basic | eco_tdcE - Escherichia coli Pyruvate Formate Lyase (Uniprot: P42632 · TDCE_ECOLI) | Escherichia coli |
| BBa_253Y2NDJ | Basic | saz_aCA - Sulfurihydrogenibium azorense alpha carbonic anhydrase (Uniprot: C1DTU5 · C1DTU5_SULAA) | Sulfurihydrogenibium azorense |
| BBa_25HL4OTA | Basic | hsa_thsaccat - Homo sapiens Adenylate cyclase type 10 (Uniprot: Q96PN6 · ADCYA_HUMAN) | Homo sapiens |
| BBa_25T3PV09 | Basic | ype_tesB - Yersinia pestisi Acyl-CoA thioesterase 2 (Uniprot: A0A3N4BCC7 · A0A3N4BCC7_YERPE) | Yersinia pestisi |
| BBa_25QFH341 | Basic | sfl_yciA - Shigella flexneri Acyl-CoA thioesterase (Uniprot: P0A8Z6 · YBGC_SHIFL) | Shigella flexneri |
| Basic | SadCas9 - Staphylococcus aureus dCas9 (Uniprot: A0A386IRG9 · A0A386IRG9_STAAU) | Staphylococcus aureus | |
| BBa_259PG5OZ | Basic | SadCas9pt1 - Staphylococcus aureus dCas9 (Uniprot: A0A386IRG9 · A0A386IRG9_STAAU) | Staphylococcus aureus |
| BBa_25JM8TAX | Basic | SadCas9pt2 - Staphylococcus aureus dCas9 (Uniprot: A0A386IRG9 · A0A386IRG9_STAAU) | Staphylococcus aureus |
| BBa_25DUAQM1 | Composite | sgRNA–gltarec-msGFP2 - a chimeric sgRNA targeting the endogenous E. coli gltA (citrate synthase) gene, plus a short gltA recognition sequence placed 5′ of msGFP2 (Aequorea victoria) to create a defined Sa-dCas9/sgRNA binding site. Occupancy of this site represses the reporter, enabling a quantitative readout of CRISPRi knockdown. | E. coli / Aequorea victoria |
| BBa_25ZOXF2L | Composite | sgRNA-sadCas9 | Staphylococcus aureus |
