KT2440 ALE

1. Pseudomonas putida KT2440 Acid Tolerance Adaptation Protocol

About KT 2440

1. Basic Procedures for Pseudomonas putida culturing and genetic manipulation

2. Genetic knockout experiment of Pseudomonas putida KT2440

3. Quantitative analysis of succinate content with a succinate assay kit

P. pastoris Pathway

1. Protocol for Pichia pastoris Competent Cell Preparation, Electroporation, and Expression

2. Protocol for Lignin Detection and Laccase Activity Assay

T. reesei Pathway

1. Basic Procedures for T. reesei culturing and genetic manipulation

2. Cellulose degradation & Coculture experiments

3. Lignin degradation of genetically modified T. reesei experiments

4. Protocol for Lignin Detection and Laccase Activity Assay

UTR Pathway

1. UTR Optimization Protocol with Fluorescence Detection