Ducon is supported by 4 integrated part collections that each perform a specific function. With every part carefully explained and completely documented, our team presents not just functional systems, but also a philosophy on what it means to be a real collection.
It is important to note that the collection submitted for judging (UUID: 83dce544-4b00-4be8-9f41-c0f2e729572f) represents a comprehensive integration of all four specialized collections listed below. This master collection combines the succinate production capabilities of Pseudomonas putida KT2440, the laccase expression systems for both Pichia pastoris and Trichoderma reesei, and the 5’ Expression Enhancer UTR optimization toolkit into a unified resource that embodies the complete DuCon engineering approach.
| Collection UUID | Collection Name | Description |
|---|---|---|
| 83dce544-4b00-4be8-9f41-c0f2e729572f | DuCon collection | The combination of the following four collections |
| 5ff3cdff-c766-44d3-8168-ddc63da5dc68 | Pseudomonas putida KT2440’s succinate production cassette | Succinate production cassette for Pseudomonas putida KT2440 |
| 1a064d82-1408-4b1a-9e05-a92f5725a3af | Pichia pastoris’s laccase expression cassette | Laccase expression cassette for Pichia pastoris |
| b29c66c1-06a5-4225-9600-dfea071fab4b | 5’ Expression Enhancer UTRs | 5’ Expression Enhancer UTR collection |
| ca6a2c81-3dd8-41e7-a5d3-31011c05fb54 | Trichoderma reesei’s laccase expression cassette | Laccase expression cassette for Trichoderma reesei |
| Plasmid ID | Plasmid Name | Basic Part ID | Basic Part Name |
|---|---|---|---|
| BBa_25T8KJD0 | pUC19-5’ Expression Enhancer UTR 11701-GFP | BBa_258WLUCR | 5’ Expression Enhancer UTR 11701 |
| BBa_25X49JUK | pUC19-GFP without 5’ Expression Enhancer UTR | ||
| BBa_25L6JCIL | pUC19-5’ Expression Enhancer UTR 8600-GFP | BBa_25FAKY7Y | 5’ Expression Enhancer UTR 8600 |
| BBa_25X49JUK | pUC19-GFP without 5’ Expression Enhancer UTR | ||
| BBa_25NTOYPO | pUC19-5’ Expression Enhancer UTR 15881-GFP | BBa_258GOE5F | 5’ Expression Enhancer UTR 15881 |
| BBa_25X49JUK | pUC19-GFP without 5’ Expression Enhancer UTR | ||
| BBa_25C3SQEV | pUC19-5’ Expression Enhancer UTR 7116-GFP | BBa_25WPZCC4 | 5’ Expression Enhancer UTR 7116 |
| BBa_25X49JUK | pUC19-GFP without 5’ Expression Enhancer UTR | ||
| BBa_25VPKE3N | pUC19-5’ Expression Enhancer UTR 15951-GFP | BBa_25AEBUFO | 5’ Expression Enhancer UTR 15951 |
| BBa_25X49JUK | pUC19-GFP without 5’ Expression Enhancer UTR |
| Plasmid ID | Plasmid Name | Composite Part ID | Composite Part Name | Basic Part ID | Basic Part Name |
|---|---|---|---|---|---|
| BBa_25AK1TAO | pAN7-1-Trichoderma reesei laccase expression cassette | BBa_258T5DUN | Trichoderma reesei laccase expression cassette | BBa_25DF7RQ7 | Trichoderma reesei TRIREDRAFT_110879 promoter |
| BBa_25QRRAP5 | Trichoderma reesei signalling peptide | ||||
| BBa_25YKL2Q7 | Trametes versicolor K4 laccase gene without signalling peptide | ||||
| BBa_25SXLKRJ | Trichoderma reesei cbh1 terminator | ||||
| BBa_25OCIRFW | Trichoderma reesei pyr4 gene | ||||
| / | / | BBa_252QLUL7 | Trichoderma reesei pAN7-1 plasmid backbone (Partial) |
| Plasmid ID | Plasmid Name | Basic Part ID | Basic Part Name | Related Primers |
|---|---|---|---|---|
| BBa_25QWD6MO | pBBR1MCS2-Tac-DcuB (KanR) | BBa_25GOD0HS | Escherichia coli dcuB (optimized for expression in Pseudomonas putida) | BBa_25HKH7S8: DcuB P1 3’; BBa_25W04ZR4: DcuB P2 3’ |
| BBa_25AW0FXV | pBBR1MCS2-Tac-mCherry plasmid, mCherry removed | BBa_255FVSTD: pBBR P1 3’; BBa_25CZ4MPJ: pBBR P2 3’ | ||
| BBa_25DJEEMW | pK18mobsacB-sdh KO plasmid | BBa_25NHOM1I | Pseudomonas putida sdh gene upstream homologous region | BBa_25CKH84S: SdhP1; BBa_257K320M: SdhP2; BBa_25LF9KF0: ppk forward primer; BBa_25NRNGQZ: ppk reverse primer |
| BBa_254T1BPA | Pseudomonas putida sdh gene downstream homologous region | |||
| BBa_25RJOKEL | pK18mobsacB with 21 bp deletions in MCS | |||
| BBa_251NITZ2 | pBBR1MCS2-Tac-FrdA (AmpR) | BBa_257T3ZAS | Escherichia coli frdA (optimized for expression in Pseudomonas putida) | BBa_25U5C06V: AmpR forward primer; BBa_25GHW5PV: AmpR reverse primer; BBa_25Z772YP: pBBR1MCS2-Tac forward primer; BBa_25FLAIIK: pBBR1MCS2-Tac reverse primer |
| BBa_25S5BUUG | pBBR1MCS2-Tac-mCherry plasmid, mCherry removed, ampicillin resistant |
| BBa_25RHMO7V | pPICK-laccase-EGFP (codons unrefined) |
| BBa_256S6J3U | pPICKαAm-T38473-eGFP, codons refined |
| BBa_25F488VT | pPICKαAm-T38473-laccase-linker-eGFP, codons refined |
| BBa_255407DG | pPICKαAm-T38473-laccase, codons refined |
For Plasmid BBa_256S6J3U
| Basic Part ID | Basic Part Name | Related Primers |
|---|---|---|
| BBa_255WV1YB | pPICKαAm-T38473 | BBa_25WDQS4O: pPICKαAm-T38473 forward primer; BBa_25F835JO: pPICKαAm-T38473 reverse primer |
| BBa_2512I90M | laccase-linker-eGFP eGFP, codons refined | BBa_250EZ8CO: αAm-EGFP forward primer; BBa_25K7V7CU: αAm-EGFP reverse primer |
For Plasmid BBa_25F488VT
| Composite Part ID | Composite Part Name | Basic Part ID | Basic Part Name | Related Primers |
|---|---|---|---|---|
| BBa_25BL25OQ | laccase-linker-eGFP, codons refined | BBa_25MV7PAJ | Trametes versicolor K4 laccase gene without signalling peptide & stop codon, codons refined | / |
| BBa_25A25LJ6 | laccase-linker-eGFP linker, codons refined | BBa_256FMAMR: laccase-linker-eGFP forward primer; BBa_25CGYIVH: laccase-linker-eGFP reverse primer | ||
| BBa_2512I90M | laccase-linker-eGFP eGFP, codons refined | |||
| / | / | BBa_255WV1YB | pPICKαAm-T38473 | BBa_25WDQS4O: pPICKαAm-T38473 forward primer; BBa_25F835JO: pPICKαAm-T38473 reverse primer |
For Plasmid BBa_25F488VT
| Basic Part ID | Basic Part Name |
|---|---|
| BBa_25MV7PAJ | Trametes versicolor K4 laccase gene without signalling peptide & stop codon, codons refined |
| BBa_255WV1YB | pPICKαAm-T38473 |
| Collection UUID | Collection Name | Sequence ID | Sequence Name |
|---|---|---|---|
| 5ff3cdff-c766-44d3-8168-ddc63da5dc68 | Pseudomonas putida KT2440’s succinate production cassette | BBa_25WKGNHN | pET22b P1 |
| BBa_25VMB1MD | pET22b P2 | ||
| BBa_25D2D9A5 | CotA P1’ Primer | ||
| BBa_25JNKGZ6 | CotA P2’ Primer | ||
| BBa_255FVSTD | pBBR P1 3’ | ||
| BBa_25CZ4MPJ | pBBR P2 3’ | ||
| BBa_25HKH7S8 | DcuB P1 3’ | ||
| BBa_25W04ZR4 | DcuB P2 3’ | ||
| BBa_25U5C06V | AmpR forward primer | ||
| BBa_25GHW5PV | AmpR reverse primer | ||
| BBa_25Z772YP | pBBR1MCS2-Tac forward primer | ||
| BBa_25FLAIIK | pBBR1MCS2-Tac reverse primer | ||
| BBa_25CKH84S | SdhP1 | ||
| BBa_257K320M | SdhP2 | ||
| BBa_25LF9KF0 | ppk forward primer | ||
| BBa_25NRNGQZ | ppk reverse primer | ||
| 1a064d82-1408-4b1a-9e05-a92f5725a3af | Pichia pastoris’s laccase expression cassette | BBa_25V9M0D5 | αAm-EGFP forward primer for homologous recombination of pPICKαAm-T38473 vector |
| BBa_259DUW68 | αAm-EGFP reverse primer for homologous recombination of pPICKαAm-T38473 vector | ||
| BBa_250EZ8CO | αAm-EGFP forward primer for homologous recombination of eGFP | ||
| BBa_25K7V7CU | αAm-EGFP reverse primer for homologous recombination of eGFP | ||
| BBa_25WDQS4O | pPICKαAm-T38473 forward primer for removal of linker-eGFP | ||
| BBa_25F835JO | pPICKαAm-T38473 reverse primer for removal of linker-eGFP | ||
| BBa_256FMAMR | laccase-linker-eGFP (condons refined) forward primer for removal of linker-eGFP | ||
| BBa_25CGYIVH | laccase-linker-eGFP (condons refined) reverse primer for removal of linker-eGFP |