Scientific research thrives on transparency and reproducibility. Our laboratory notebook serves as a comprehensive chronicle of our experimental journey, documenting every protocol, observation, success, and setback encountered throughout the project. This detailed record not only ensures the traceability of our results but also provides valuable insights for future teams building upon our work. Here you will find our day-to-day laboratory activity that contributed to shaping HERO and HELMET project, advancing our understanding of Rhodococcus opacus as a chassis for bioremediation.