Parts Overview

Building the future of synthetic biology, one part at a time.

Overview

We submitted 37 well-documented BioBrick parts, including all those used in our project. This collection comprises 14 basic parts (see Basic Parts page) and 23 composite parts (see Composite parts page).

Basic Parts

Registry Code	Part Name	Function	Length (bp)
BBa_25J460FA⤴	TMD	Encodes the transmembrane domain of the CD4 glycoprotein, enabling stable anchoring of fusion proteins to the plasma or vesicle membrane.	77
BBa_25Q8SCNI	Notchcore	Encodes the transmembrane core domain of the Notch receptor, mediating membrane integration and potential receptor-like topology for fusion constructs.	978
BBa_252HSMKK⤴	CAAX membrane anchor	Encodes the CAAX prenylation motif, directing attached proteins to the inner leaflet of the plasma membrane via lipid modification.	45
BBa_25FPJ465⤴	LifeAct	Encodes a 17–amino acid peptide that specifically binds filamentous actin (F-actin) without interfering with actin polymerization dynamics, enabling cytoskeletal localization of fusion proteins.	51
BBa_255Z07L1⤴	KDEL	Encodes a C-terminal retention motif that retains soluble proteins in the endoplasmic reticulum through the KDEL receptor retrieval pathway.	12
BBa_25Q2M02J⤴	Mitochondrial Targeting Sequence (MTS)	Encodes an N-terminal mitochondrial signal peptide that directs fusion proteins to the mitochondrial matrix or inner membrane.	75
BBa_250O67UT⤴	Kozak sequence	Provides a consensus sequence surrounding the start codon to enhance ribosomal recognition and translation initiation efficiency in mammalian cells.	10
BBa_258ZYB5B⤴	IgK–Nluc	Encodes a secreted form of NanoLuc luciferase driven by the Igκ signal peptide, serving as a luminescent reporter for secretion assays.	597
BBa_25WDV48R⤴	mMaple3	Encodes a photoconvertible fluorescent protein responsive to blue–violet light, used as a light-sensitive regulatory element or reporter.	711
BBa_25K5NJ9H⤴	Furin Site	Encodes a protease recognition sequence cleaved by endogenous furin during vesicle trafficking, enabling post-Golgi release of cargo proteins.	15
BBa_25RHALHC⤴	CarH	Encodes a green-light–responsive sensor protein that dissociates from oligomers into monomers upon illumination, allowing optogenetic control of protein–protein interactions.	615
BBa_25HYFBUH⤴	NS3a	Encodes the NS3/4A serine protease domain that forms reversible complexes with ANR or GNCR proteins, functioning as a drug-responsive protein–protein interaction switch activated by Grazoprevir.	588
BBa_25CQAUVL⤴	Apo NS3a leader (ANR)	Encodes a high-affinity protein partner that binds NS3a in the absence of Grazoprevir and dissociates rapidly upon drug treatment, enabling small-molecule–controlled complex disruption.	63
BBa_25DRNV7I⤴	GNCR	Encodes a high-affinity protein interacting with NS3a in a Grazoprevir-dependent manner, serving as an alternative small-molecule–responsive binding partner.	699

Composite Parts

Registry Code	Part Name	Function	Length (bp)
BBa_25R1OZ0W⤴	P_CMV-IgK-Nluc-FS-TMD-mCherry-BGHPA	Expresses a constitutively secreted NanoLuc luciferase fused to a transmembrane anchor and mCherry reporter, serving as an unregulated control for secretion efficiency.	1167
BBa_250EVBWI⤴	P_CMV-IgK-Nluc-FS-TMD-mCherry-LifeAct-BGHPA	Encodes NanoLuc tethered to the cytoskeleton via LifeAct for visualization and analysis of vesicle retention independent of external signals.	1880
BBa_25RPYT7R⤴	P_CMV-Lifeact-CarH-BGHPA	Produces a cytoskeleton-anchored, green-light–sensitive CarH fusion protein used to control protein interactions via light-induced dissociation.	1110
BBa_252RTW6P⤴	P_CMV-IgK-Nulc-FS-TMCD4-_2GSlinker-CarH-BGHPA	Encodes a secreted NanoLuc fusion linked to the CarH photosensor, allowing light-dependent vesicle release and secretion.	1746
BBa_25NHFT5Q⤴	P_CMV-IgK-Nluc-FS-TMCD4-BphP1-BGHPA	Expresses a secreted NanoLuc fused to the near-infrared light sensor BphP1 for red/NIR-controlled protein release.	3327
BBa_25LJBOAU⤴	P_CMV-Lifeact-BphP1-BGHPA	Encodes a LifeAct-anchored BphP1 phytochrome fusion for cytoskeletal localization and optogenetic control under far-red illumination.	2704
BBa_25TK8QFS⤴	P_CMV-PpsR2-BGHPA	Expresses the far-red light-responsive transcriptional regulator PpsR2 to evaluate its interaction dynamics with BphP1.	1786
BBa_25NYLBFF⤴	P_CMV-ANR-mCherry-LifeAct-BGHPA	Encodes a red fluorescent ANR fusion anchored to the cytoskeleton, enabling small-molecule-controlled association and dissociation with NS3a.	1264
BBa_25WEPAKX⤴	P_CMV-IgK-(Nluc-FS)_3-TMD-NS3a_2-BGHPA	Encodes a secretory NanoLuc fusion bearing tandem NS3a domains for drug-inducible interaction with ANR and rapid, Grazoprevir-triggered secretion.	3411
BBa_2506II6Z⤴	P_CMV-IgK-Fluc-TMD-NS3a-BGHPA	Produces a membrane-anchored firefly luciferase fusion containing the NS3a domain for small-molecule–responsive secretion assays.	2751
BBa_2596NLG0⤴	P_CMV-ANR-TetR-NLS-VP64-BGHPA	Encodes a modular transcriptional activator combining ANR-TetR interaction, nuclear localization, and VP64 activation domain for drug-responsive gene expression.	1339
BBa_25EPQC3P⤴	TCE-IgK-Nluc-BGHPA	Drives expression of secreted NanoLuc under a Tet-controlled element (TCE) promoter to evaluate transcriptional activation efficiency in small-molecule systems.	1165
BBa_25PW9O16⤴	P_CMV-GNCR-GSlinker-NLS-VP64- BGHPA	Produces a GNCR-based nuclear transcriptional activator responsive to NS3a interaction, enabling signal-controlled reporter gene induction.	1327
BBa_25NDCL0K⤴	P_CMV -TetR-NLS-NS3a-BGHPA	Encodes a TetR-NS3a fusion localized to the nucleus, forming part of the drug-controlled transcriptional activation system.	1735
BBa_25QZZZ22⤴	P_CMV-IgK-(Nluc-FS)_2-TMD-NS3a_2-BGHPA	Expresses a dual-copy NanoLuc fusion with two NS3a domains for optimized signal responsiveness and enhanced secretion control.	2880
BBa_25B4NQ85⤴	P_CMV-IgK-Nluc-FS-TMD-NS3a_2-BGHPA	Encodes a single-NanoLuc fusion containing two NS3a units to balance basal retention and drug-induced secretion sensitivity.	2349
BBa_25UUGKON⤴	P_CMV-IgK-Nluc-FS-TMD-NS3a-BGHPA	Expresses a secretory NanoLuc coupled with a single NS3a domain, enabling basic evaluation of small-molecule-triggered release kinetics.	1719
BBa_25YVM5DM⤴	P_CMV-IgK-Nluc-FS-TMD-NS3a_3-BGHPA	Encodes a NanoLuc fusion bearing three NS3a domains to investigate multivalent effects on vesicle retention and response amplitude.	2979
BBa_25M95JXL⤴	P_CMV-EGFP-TMD-LifeAct-BGHPA	Produces a green fluorescent fusion protein anchored to the cytoskeleton, enabling visualization of vesicle immobilization.	1302
BBa_2578SBJ3⤴	P_CMV-LifeAct-mCherry-BGHPA	Expresses a red fluorescent LifeAct fusion for real-time visualization of F-actin organization and anchoring behavior.	559
BBa_25IZYJLX⤴	P_CMV-EGFP-TMD-BGHPA	Encodes a membrane-localized green fluorescent protein serving as a reference for plasma-membrane targeting efficiency.	1230
BBa_25W216DF⤴	P_CMV-IgK-Nluc- BGHPA	Expresses a secreted NanoLuc luciferase as a baseline control for secretion assays.	1033
BBa_25WLRLM7⤴	P_CMV-IgK-Nluc-TMD-mMaple3-LifeAct-BGHPA	Produces a cytoskeleton-anchored, blue-violet–light–regulated NanoLuc fusion for photolytically controlled protein secretion.	1950